ML851 pSP6-nsP1-nsP3 (with ALI mutations)-GFP-MEK1-P2A-PuroR
- Purpose The plasmid is used for MEK1 evolution under drugs treatment.
- Orthogonal RNA replication enables directed evolution and Darwinian adaptation in mammalian cells
- Depositing Lab Yihan Lin Lab
- Availability Not available to industry
- MTA Required
-
Backbone
Vector:
IVT
Expression:
Mammalian expression
Promoter:
pSP6
Copy number:
High copy
-
Gene/Insert
Gene Names:
nsP1, nsP2, nsP3, EGFP, MEK1, PuroR
Species:
synthetic, synthetic, synthetic, synthetic, human, synthetic
Codon optimization:
None
-
Growth in bacteria
E. coli Strain:
DH5a
Antibiotic:
AmpR
Growth Condition:
37⁰C
-
Virus samples
Virus samples:
Not available
Transgenic animals:
Not available
