pKSEe401R

0000445 (Barcode)

• PurposeCRISPR/Cas9 genome editing vector for dicots. pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone. NOTE: as pKSEe401R is the modified version of original pKSE401 pKSE401 [Xing et al., 2014. BMC Plant Biology. 14: 1-12], it allows constructing vectors expressing ONLY one gRNA for gene targeting. You may use original pCBC-DT1T2, pCBC-DT2T3, and pCBC-DT3T4 (AddGene IDs: 50590, 50591, 50592, respectively) for construction two-, three-, or four-gRNA-expressing vectors. PROPAGATION: use kanamycin (50 mkg/ml) together with spectinomycin (50 mkg/ml) for empty plasmid propagation both in E. coli and in Agrobacterium strains. Spectinomycin allows saving the pressure against BsaI-containing cassette.
• Publication View Article All Plasmids from Article
  Do DEEPER ROOTING 1 homologs regulate the lateral root slope angle in cucumber (Cucumis sativus)?
• Depositing Lab Demchenko Lab (BIN RAS)
• AvailabilityNot available to industry
• MTANot required