pKSEe401R
- Purpose CRISPR/Cas9 genome editing vector for dicots. pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone. NOTE: as pKSEe401R is the modified version of original pKSE401 pKSE401 [Xing et al., 2014. BMC Plant Biology. 14: 1-12], it allows constructing vectors expressing ONLY one gRNA for gene targeting. You may use original pCBC-DT1T2, pCBC-DT2T3, and pCBC-DT3T4 (AddGene IDs: 50590, 50591, 50592, respectively) for construction two-, three-, or four-gRNA-expressing vectors. PROPAGATION: use kanamycin (50 mkg/ml) together with spectinomycin (50 mkg/ml) for empty plasmid propagation both in E. coli and in Agrobacterium strains. Spectinomycin allows saving the pressure against BsaI-containing cassette.
- Do DEEPER ROOTING 1 homologs regulate the lateral root slope angle in cucumber (Cucumis sativus)?
- Depositing Lab Demchenko Lab (BIN RAS)
- Availability Not available to industry
- MTA Not required
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Backbone
Vector:
pKSE401
Expression:
Plant
Promoter:
p35S, pAtUBQ10, pAtU6-26
Copy number:
High copy
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Gene/Insert
Gene Names:
OsMac3 5-UTR fragment, pAtUBQ10-DsRED1-NOSt cassette
Species:
Plants
Codon optimization:
Plants
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Growth in bacteria
E. coli Strain:
DH5a, XL1Blue, TOP10
Antibiotic:
KanamycinSpectinomycin
Growth Condition:
37⁰C
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Virus samples
Virus samples:
Not Available
Transgenic animals:
Not Available
