pAAV-AscI-CAG-H2B-Nelle-L3-mCherry
- Purpose Nuclear expression of green Nelle Isoleucin sensor and mCherry in mammalian cells
- NeIle, a Genetically Encoded Indicator for Branched-Chain Amino Acids Based on mNeonGreen Fluorescent Protein and LIVBP Protein
- Depositing Lab Laboratory of Molecular Design
- Availability Available to everyone
- MTA Not required
-
Backbone
Vector:
pAAV-CAG
Expression:
Mammalian expression
Promoter:
CAG
Copy number:
High copy
-
Gene/Insert
Gene Names:
H2B, Nelle, mCherry
Species:
Human, Synthetic, Synthetic
Codon optimization:
None
-
Growth in bacteria
E. coli Strain:
DH5a, TOP10
Antibiotic:
Ampicillin
Growth Condition:
37℃
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Virus samples
Virus samples:
Not Available
Transgenic animals:
Not Available
