SuperFiCas9-tRNA-gRNA-all-in-One
- Purpose Mammalian expression of Cas9 and sgRNA scaffold
- Spacer Extension Restores Cleavage Activity by Enhancing PAM-Distal Interactions in High-Fidelity Cas9
- Depositing Lab Westlake Genetech
- Availability Available to everyone
- MTA Required
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Backbone
Vector:
LV-SFCas9
Expression:
Lenti, Mammalian expression
Promoter:
CMV, U6
Copy number:
High copy
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Gene/Insert
Gene Names:
superFiCas9, GFP
Species:
H.sapiens
Codon optimization:
None
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Growth in bacteria
E. coli Strain:
Stbl3
Antibiotic:
Ampicillin
Growth Condition:
37℃
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Virus samples
Virus samples:
Not available
Transgenic animals:
Not available
