pOJ260
- Purpose Plasmid pOJ260 is an integrative cloning vector commonly used in actinomycetes such as *Streptomyces*. Its applications include gene expression and functional studies in actinomycetes like *Streptomyces*; cloning and heterologous expression of biosynthetic gene clusters for secondary metabolites (e.g., antibiotics); and achieving single-copy or multi-copy gene integration using the φC31 integration system.
- Development of a thiostrepton-free system for stable production of PLD in Streptomyces lividans SBT5
- Depositing Lab Hunan Provincial Key Laboratory for Microbial Molecular Biology
- Availability Available to everyone
- MTA Required
-
Backbone
Vector:
pOJ260
Expression:
Bacterial expression
Promoter:
ermE
Copy number:
Medium copy
-
Gene/Insert
Gene Names:
none insert gene
Species:
saccharopolyspora spinosa
Codon optimization:
none
-
Growth in bacteria
E. coli Strain:
PUZ8002
Antibiotic:
ErmE
Growth Condition:
37
-
Virus samples
Virus samples:
Not Available
Transgenic animals:
Not Available
