pKGW-DR-MGW
- Purpose Destination vector for multiple Gateway cloning by attR4 and attR3 recombination sites. pKGW-DR-MGW can be used for the localization of promoter-reporter or translational fusions together with visualization of cellular auxin response maxima (DR5-mRuby3-H2B). NOTE: you need to use three different entry vectors (one with attL4 and attR1 recombination sites, one with attL1 and attL2, and one with attR2 and attL3). PROPAGATION: use spectinomycin (50 mkg/ml) together with the chloramphenicol (30 mkg/ml) for empty plasmid propagation both in E. coli and in Agrobacterium strains. Chloramphenicol allows saving the pressure against ccdB cassette.
- Lateral root initiation in cucumber (Cucumis sativus): what does the expression pattern of Rapid Alkalinization Factor 34 (RALF34) tell us?
- Depositing Lab Demchenko Lab (BIN RAS)
- Availability Not available to industry
- MTA Not required
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Backbone
Vector:
pKGW-MGW
Expression:
Plant
Promoter:
pNOS, DR5
Copy number:
Unknown copy
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Gene/Insert
Gene Names:
DR5-mRuby3-H2B-AtACT2t cassette
Species:
Plants
Codon optimization:
Plants
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Growth in bacteria
E. coli Strain:
CcdB survival
Antibiotic:
SpectinomycinChloramphenicol
Growth Condition:
37℃
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Virus samples
Virus samples:
Not Available
Transgenic animals:
Not Available
