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'"	HKx#2Lk4Rau 1pk			4
?UkS_P}+ m lI	9	S		
xz=4   (_U0G^`	t	.



LtvWY N8di3  vie?	J	]		






lq,p{ B[en     ?h*5W5	(i   IN<i
  `&
0
3~	6 4{rP	v	
N? X "]V
	

@n ELe@~	$ 7 =  )Yq?< ^OUluB		b


/Lap "  B):		
 S   C:--			}" ) I uxAa#>
P
u
)eC8S%D-}: F"			B


&TjGV}Rw1TixD]qW}3`t0 l '^>g)V >	_	s	-





 kps+o'Ah<m 3Hz;O	


?5> ! < R  Ad>(,]9Tk,	A	}				
.`L k 2t"!	N		,
t






%Si(>o|*BQnv &0h7Rg$lp|2=_s| Q \ i j  01@s$%&'d|L	M		


+
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s













#$Kaw=n()rstu/^_6kxyz{"#$%&1;<BCDKLM^qrxyz{, ]  xfeg}	


Lbxt'7EN y	
% % 7!=h~				
Mcy7"%FU   f
 i=u(8Odg ^ 	gF	y	
  zj
 /	
 q dy7
 YZ   R;x	*"   j86Q/3		
Ndz#GV & >	8  0
&"i		
& 7p&		k  T_g	$
>)Ph"  O  A:b7l


!&lrv/9eo  Oj
j _ ,
 
A
 o%ZG	z	T hu  Zk  k	  K.H


 {)    e 	hE
	
) /  I-G	u a 0LR z	 r 
  ed	 P8 X>  !@	 P3~ u[ %Z  wO`s		:
BXn   S<|7B0	+8 &y	Iq  cf4F k970R4Y		dE%   )	%n		7G]sCb$[ Q,0bl	(

-#HW C	Oe{   V    +2
~
59,  ' F?z	
T3 M #$	 
 P	'  '#j	8

  47\]j
[ 1 8qR' m_l  qt'	lV
 		 4" H
	 U`}	

?N*Qi  uT4]	I	R	V	 !h%
	 6 B9;Na
 H (U`8   P [ ~  =r\c8 	|	m



"Rh~'Pm]sw
!0:A]p 6	x		
$f x  
   5Pw 6^i
k
kq. tS	 -G	 ` d  gK
 ~		
 W	 %_E BXB   3\i7

 p&lU H	
 Z '[	{	 wp fJF $ kv
	 eij  %  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                               
                                                                                                                                             
   	                                                                                                                          
                        	      "   `            )   >         :   %                     
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                   
                                                         
 	                                          
        	  " `    ) >   : %   ]  ]  ]  ]  _2  aN  e1p3;fL	[		L pL 6 v :eKxKxKx#~ Q.Uoo'^_	{9 f Q)	H^t4:e	NE
x$;5PWk	@,,{4a {|/W		LWpW|hp	c4F:(		 (			 7:Q3T&_ AW5;Pc[	
y cb  1|4
gLX7  7Ol~	b

Mcy/La"FUp&_l!be3FT:0be3![d7	(}	!be3p&_lFL "#^k% # wlPJ	
H  w`lyq]j1sOY >D	g0_  Rj;p&	*k	sa	0o		
^|;Uf	N	0He> a		D	g]jyq />% =	7	X>W Y M2} r[_

" }$?H
	h.MX`|-G  | |rg[	_

" V m      (_l+U0GI^	9	S	`	t		.
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BXn: g bDRs>*	q				)

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yN[idiJyF4Bid;.{C/sar>=~i/Z̎7W"Euem.7vva0\+o6OkY\Bܗ/	i[Gf4g3ԧgdYq@J4Tƫ!WUt<7qHކ {.-橹V"?U"&W	6#oP^P]X!_6u$vx#~1;'3|f{moqQ-l%PutLK̮
(H~ʞH"9G&S2 0DaZ	t`s=+edhb1HsD8>l"n㐨UfܵO,z18r"$Ҙ:バ>'    7A_ $ *r-g    YZ  h      j  ?        ?N f+i^D6ꍟj,@QYyJݑR͛rWefTF5B`     >  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="5"><Feature recentID="4" name="promoter by ermB FROM pMT494" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="78-300" color="#a6acb3" type="standard"/></Feature><Feature recentID="3" name="RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="287-295" color="#a6acb3" type="standard"/></Feature><Feature recentID="2" name="putative promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-174" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="H1-Nanoluc" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="301-319" color="#ffff99" type="standard"/></Feature><Feature recentID="0" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-20" color="#a6acb3" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="pMT494-R" sequence="cttccagagtaaaaaccattataacctctccttaatttattgcatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="273-318" boundStrand="1" annealedBases="cttccagagtaaaaaccattataacctctccttaatttattgcatc" meltingTemperature="62"><Component hybridizedRange="273-318" bases="cttccagagtaaaaaccattataacctctccttaatttattgcatc"/></BindingSite><BindingSite simplified="1" location="273-318" boundStrand="1" annealedBases="cttccagagtaaaaaccattataacctctccttaatttattgcatc" meltingTemperature="62"><Component hybridizedRange="273-318" bases="cttccagagtaaaaaccattataacctctccttaatttattgcatc"/></BindingSite></Primer><Primer recentID="1" name="pMT494-F" sequence="TATCAGCTCACTCAAAGGCGgtggtataatatctttgttcattag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-44" boundStrand="0" annealedBases="TATCAGCTCACTCAAAGGCGgtggtataatatctttgttcattag" meltingTemperature="65"><Component hybridizedRange="0-44" bases="TATCAGCTCACTCAAAGGCGgtggtataatatctttgttcattag"/></BindingSite><BindingSite simplified="1" location="0-44" boundStrand="0" annealedBases="TATCAGCTCACTCAAAGGCGgtggtataatatctttgttcattag" meltingTemperature="65"><Component hybridizedRange="0-44" bases="TATCAGCTCACTCAAAGGCGgtggtataatatctttgttcattag"/></BindingSite></Primer></Primers>
  *<Notes>
<UUID>07b9ddf1-b4e7-4892-9d36-66f8dac0b4b6</UUID>
<Type>Natural</Type>
<Created UTC="9:33:9">2022.11.21</Created>
<LastModified UTC="10:49:44">2022.11.21</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
        e  +        + f+i^D6ꍟj,@QYyJݑR͛rWefTF5B`      *  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  @<?xml version="1.0"?><Features nextValidID="4"><Feature recentID="3" name="H1-Nanoluc" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="281-299" color="#ffff99" type="standard"/></Feature><Feature recentID="2" name="putative promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="105-154" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="267-275" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="promoter by ermB FROM pMT494" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="58-280" color="#a6acb3" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="pMT494-R" sequence="cttccagagtaaaaaccattataacctctccttaatttattgcatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="253-298" boundStrand="1" annealedBases="cttccagagtaaaaaccattataacctctccttaatttattgcatc" meltingTemperature="62"><Component hybridizedRange="253-298" bases="cttccagagtaaaaaccattataacctctccttaatttattgcatc"/></BindingSite><BindingSite simplified="1" location="253-298" boundStrand="1" annealedBases="cttccagagtaaaaaccattataacctctccttaatttattgcatc" meltingTemperature="62"><Component hybridizedRange="253-298" bases="cttccagagtaaaaaccattataacctctccttaatttattgcatc"/></BindingSite></Primer><Primer recentID="1" name="pMT494-F" sequence="TATCAGCTCACTCAAAGGCGgtggtataatatctttgttcattag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="gtggtataatatctttgttcattag" meltingTemperature="50"><Component bases="TATCAGCTCACTCAAAGGCG"/><Component hybridizedRange="0-24" bases="gtggtataatatctttgttcattag"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="gtggtataatatctttgttcattag" meltingTemperature="50"><Component bases="TATCAGCTCACTCAAAGGCG"/><Component hybridizedRange="0-24" bases="gtggtataatatctttgttcattag"/></BindingSite></Primer></Primers>
  *<Notes>
<UUID>07b9ddf1-b4e7-4892-9d36-66f8dac0b4b6</UUID>
<Type>Natural</Type>
<Created UTC="9:33:9">2022.11.21</Created>
<LastModified UTC="10:49:32">2022.11.21</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  
      g  1        1 f+i^D6ꍟj,@QYyJݑR͛rWefTF5B`Q       0  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="3"><Feature recentID="2" name="putative promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="105-154" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="267-275" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="promoter by ermB FROM pMT494" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="58-280" color="#a6acb3" type="standard"/></Feature></Features>
  Z<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="pMT494-R" sequence="cttccagagtaaaaaccattataacctctccttaatttattgcatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="253-298" boundStrand="1" annealedBases="cttccagagtaaaaaccattataacctctccttaatttattgcatc" meltingTemperature="63"><Component hybridizedRange="253-298" bases="cttccagagtaaaaaccattataacctctccttaatttattgcatc"/></BindingSite><BindingSite simplified="1" location="253-298" boundStrand="1" annealedBases="cttccagagtaaaaaccattataacctctccttaatttattgcatc" meltingTemperature="63"><Component hybridizedRange="253-298" bases="cttccagagtaaaaaccattataacctctccttaatttattgcatc"/></BindingSite></Primer><Primer recentID="1" name="pMT494-F" sequence="gtggtataatatctttgttcattag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="gtggtataatatctttgttcattag" meltingTemperature="49"><Component hybridizedRange="0-24" bases="gtggtataatatctttgttcattag"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="gtggtataatatctttgttcattag" meltingTemperature="49"><Component hybridizedRange="0-24" bases="gtggtataatatctttgttcattag"/></BindingSite></Primer></Primers>
  *<Notes>
<UUID>07b9ddf1-b4e7-4892-9d36-66f8dac0b4b6</UUID>
<Type>Natural</Type>
<Created UTC="9:33:9">2022.11.21</Created>
<LastModified UTC="10:48:36">2022.11.21</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  
       `           f+i^D6ꍟj,@QYyJݑR͛rWefTF5B        <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="3"><Feature recentID="2" name="putative promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="105-154" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="267-275" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="promoter by ermB FROM pMT494" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="58-280" color="#a6acb3" type="standard"/></Feature></Features>
  p<?xml version="1.0"?><Primers nextValidID="2"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="pMT494-R" sequence="caaaatcttccagagtaaaaaccattataacctctccttaatttattgcatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="253-279" boundStrand="1" annealedBases="tataacctctccttaatttattgcatc" meltingTemperature="53"><Component bases="caaaatcttccagagtaaaaaccat"/><Component hybridizedRange="253-279" bases="tataacctctccttaatttattgcatc"/></BindingSite><BindingSite simplified="1" location="253-279" boundStrand="1" annealedBases="tataacctctccttaatttattgcatc" meltingTemperature="53"><Component bases="caaaatcttccagagtaaaaaccat"/><Component hybridizedRange="253-279" bases="tataacctctccttaatttattgcatc"/></BindingSite></Primer><Primer recentID="1" name="pMT494-F" sequence="gtggtataatatctttgttcattag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="gtggtataatatctttgttcattag" meltingTemperature="49"><Component hybridizedRange="0-24" bases="gtggtataatatctttgttcattag"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="gtggtataatatctttgttcattag" meltingTemperature="49"><Component hybridizedRange="0-24" bases="gtggtataatatctttgttcattag"/></BindingSite></Primer></Primers>
  *<Notes>
<UUID>07b9ddf1-b4e7-4892-9d36-66f8dac0b4b6</UUID>
<Type>Natural</Type>
<Created UTC="9:33:9">2022.11.21</Created>
<LastModified UTC="10:48:12">2022.11.21</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  *                `Q(1G
eR@%;EsƴͲ1Zm'>}XVU,(q޵
n	h`Řhy/&6*UF-YFLi>82mpp(213oez:ZXeuT岛ymZ:疏%)m       s  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  	<?xml version="1.0"?><Features nextValidID="8"><Feature recentID="7" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="605-628" color="#ff9900" type="standard"/></Feature><Feature recentID="6" name="Terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="560-611" color="#ffffff" type="standard"/><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q><Q name="label"><V text="Terminator"/></Q></Feature><Feature recentID="5" name="NanoLuc" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-516" color="#a6acb3" type="standard"/><Q name="note"><V text="/Transferred_From=pD881-SR:145030 PCR Product"/><V text="/Transferred_Similarity=100.00%"/></Q><Q name="gene"><V text="NanoLuc"/></Q><Q name="label"><V text="NanoLuc"/></Q></Feature><Feature recentID="4" name="NL4" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-146" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL4"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="3" name="NL3" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="102-121" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL3"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="2" name="NL2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-91" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q><Q name="label"><V text="NL2"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="1" name="NL1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="47-66" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL1"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="0" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="629-648" color="#a6acb3" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="13"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="170_mini CTX1-R6K-F" sequence="CGTTCCACTGAGCGTCAGACC"><BindingSite location="35-44" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="35-44" bases="GCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="35-44" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="35-44" bases="GCGTCAGACC"/></BindingSite></Primer><Primer recentID="1" name="Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="604-647" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="604-647" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="604-647" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="604-647" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="2" name="Nanoluc-F" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="ctcctgcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="ctcctgcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="3" name="Nanoluc-F2" sequence="gcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="4" name="Nanoluc-Fa" sequence="caaaatagtgaggaggatatatatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="5" name="Nanoluc-F2a" sequence="atggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="6" name="976_Nanoluc-R" sequence="ggaagataggcaattagtagaagc"><BindingSite location="604-627" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="604-627" bases="ggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="604-627" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="604-627" bases="ggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="7" name="1289_Nanoluc-F2" sequence="gcagtaaaggagaaaattttatggtttttactctggaagattttg"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="8" name="1290_Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"><BindingSite location="604-647" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="604-647" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="604-647" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="604-647" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="9" name="1399_4.5sRNA-backbone-F" sequence="tgaactgcacttgctttgat"><BindingSite location="516-535" boundStrand="0" annealedBases="tgaactgcacttgctttgat" meltingTemperature="53"><Component hybridizedRange="516-535" bases="tgaactgcacttgctttgat"/></BindingSite><BindingSite simplified="1" location="516-535" boundStrand="0" annealedBases="tgaactgcacttgctttgat" meltingTemperature="53"><Component hybridizedRange="516-535" bases="tgaactgcacttgctttgat"/></BindingSite></Primer><Primer recentID="10" name="1360_Nanoluc-F2a" sequence="atggtttttactctggaagattttg"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="11" name="1361_Overlap-nanoluc-R" sequence="CTGATTCTGTGGATAACCGTATTAC"><BindingSite location="628-652" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="54"><Component hybridizedRange="628-652" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite><BindingSite simplified="1" location="628-652" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="54"><Component hybridizedRange="628-652" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite></Primer><Primer recentID="12" name="975_Nanoluc-F" sequence="caaaatagtgaggaggatatatatggtttttactctggaagattttg"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>729d5059-0ad3-4e40-ae16-c1d26ed2209d</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="10:59:41">2022.11.21</Created>
<LastModified UTC="10:59:41">2022.11.21</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  *   
             `Q(1G
eR@%;EsƴͲ1Zm'>}XVU,(q޵
n	h`Řhy/&6*UF-YFLi>82mpp(213oez:ZXeuT岛ymZ:疏%)      s  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  	<?xml version="1.0"?><Features nextValidID="8"><Feature recentID="7" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="605-628" color="#ff9900" type="standard"/></Feature><Feature recentID="6" name="Terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="560-611" color="#ffffff" type="standard"/><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q><Q name="label"><V text="Terminator"/></Q></Feature><Feature recentID="5" name="NanoLuc" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-516" color="#a6acb3" type="standard"/><Q name="note"><V text="/Transferred_From=pD881-SR:145030 PCR Product"/><V text="/Transferred_Similarity=100.00%"/></Q><Q name="gene"><V text="NanoLuc"/></Q><Q name="label"><V text="NanoLuc"/></Q></Feature><Feature recentID="4" name="NL4" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-146" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL4"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="3" name="NL3" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="102-121" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL3"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="2" name="NL2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-91" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q><Q name="label"><V text="NL2"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="1" name="NL1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="47-66" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL1"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="0" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="629-648" color="#a6acb3" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="13"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="975_Nanoluc-F" sequence="caaaatagtgaggaggatatatatggtttttactctggaagattttg"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="1" name="1361_Overlap-nanoluc-R" sequence="CTGATTCTGTGGATAACCGTATTAC"><BindingSite location="628-647" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTAC" meltingTemperature="50"><Component bases="CTGAT"/><Component hybridizedRange="628-647" bases="TCTGTGGATAACCGTATTAC"/></BindingSite><BindingSite simplified="1" location="628-647" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTAC" meltingTemperature="50"><Component bases="CTGAT"/><Component hybridizedRange="628-647" bases="TCTGTGGATAACCGTATTAC"/></BindingSite></Primer><Primer recentID="2" name="1360_Nanoluc-F2a" sequence="atggtttttactctggaagattttg"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="3" name="1399_4.5sRNA-backbone-F" sequence="tgaactgcacttgctttgat"><BindingSite location="516-535" boundStrand="0" annealedBases="tgaactgcacttgctttgat" meltingTemperature="53"><Component hybridizedRange="516-535" bases="tgaactgcacttgctttgat"/></BindingSite><BindingSite simplified="1" location="516-535" boundStrand="0" annealedBases="tgaactgcacttgctttgat" meltingTemperature="53"><Component hybridizedRange="516-535" bases="tgaactgcacttgctttgat"/></BindingSite></Primer><Primer recentID="4" name="1290_Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"><BindingSite location="604-647" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="604-647" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="604-647" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="604-647" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="5" name="1289_Nanoluc-F2" sequence="gcagtaaaggagaaaattttatggtttttactctggaagattttg"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="6" name="976_Nanoluc-R" sequence="ggaagataggcaattagtagaagc"><BindingSite location="604-627" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="604-627" bases="ggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="604-627" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="604-627" bases="ggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="7" name="Nanoluc-F2a" sequence="atggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="8" name="Nanoluc-Fa" sequence="caaaatagtgaggaggatatatatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="9" name="Nanoluc-F2" sequence="gcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="10" name="Nanoluc-F" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="ctcctgcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="ctcctgcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="11" name="Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="604-647" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="604-647" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="604-647" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="604-647" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="12" name="170_mini CTX1-R6K-F" sequence="CGTTCCACTGAGCGTCAGACC"><BindingSite location="35-44" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="35-44" bases="GCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="35-44" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="35-44" bases="GCGTCAGACC"/></BindingSite></Primer></Primers>
  +<Notes>
<UUID>5ccd2f1c-f879-4f29-a454-5e891d37e773</UUID>
<Type>Natural</Type>
<Created UTC="14:13:4">2022.10.24</Created>
<LastModified UTC="10:59:30">2022.11.21</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  "q   	             *jh2jԾ N+~!23l/EVkboVձnK.JmBis"fjɅʨpQ|'`Q._O+̇\"
Laxގ]nU7l#F[Vg
ot      t  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  	<?xml version="1.0"?><Features nextValidID="8"><Feature recentID="7" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="606-629" color="#ff9900" type="standard"/></Feature><Feature recentID="6" name="Terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="561-612" color="#ffffff" type="standard"/><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q><Q name="label"><V text="Terminator"/></Q></Feature><Feature recentID="5" name="NanoLuc" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2-517" color="#a6acb3" type="standard"/><Q name="note"><V text="/Transferred_From=pD881-SR:145030 PCR Product"/><V text="/Transferred_Similarity=100.00%"/></Q><Q name="label"><V text="NanoLuc"/></Q><Q name="gene"><V text="NanoLuc"/></Q></Feature><Feature recentID="4" name="NL4" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="128-147" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL4"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="3" name="NL3" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="103-122" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL3"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="2" name="NL2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="73-92" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q><Q name="label"><V text="NL2"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="1" name="NL1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="48-67" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL1"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="0" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="630-649" color="#a6acb3" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="8" name="1290_Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"><BindingSite location="605-648" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="605-648" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="605-648" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="605-648" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="7" name="1289_Nanoluc-F2" sequence="gcagtaaaggagaaaattttatggtttttactctggaagattttg"><BindingSite location="0-25" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaattt"/><Component hybridizedRange="0-25" bases="tatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-25" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaattt"/><Component hybridizedRange="0-25" bases="tatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="0" name="976_Nanoluc-R" sequence="ggaagataggcaattagtagaagc"><BindingSite location="605-628" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="605-628" bases="ggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="605-628" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="605-628" bases="ggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="1" name="Nanoluc-F2a" sequence="atggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1-25" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="1-25" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="1-25" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="1-25" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="2" name="Nanoluc-Fa" sequence="caaaatagtgaggaggatatatatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-25" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatata"/><Component hybridizedRange="0-25" bases="tatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-25" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatata"/><Component hybridizedRange="0-25" bases="tatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="3" name="Nanoluc-F2" sequence="gcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-25" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaattt"/><Component hybridizedRange="0-25" bases="tatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-25" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="gcagtaaaggagaaaattt"/><Component hybridizedRange="0-25" bases="tatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="4" name="Nanoluc-F" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-25" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="ctcctgcagtaaaggagaaaattt"/><Component hybridizedRange="0-25" bases="tatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-25" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="ctcctgcagtaaaggagaaaattt"/><Component hybridizedRange="0-25" bases="tatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="5" name="Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="605-648" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="605-648" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="605-648" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="605-648" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="6" name="170_mini CTX1-R6K-F" sequence="CGTTCCACTGAGCGTCAGACC"><BindingSite location="36-45" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="36-45" bases="GCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="36-45" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="36-45" bases="GCGTCAGACC"/></BindingSite></Primer></Primers>
  *<Notes>
<UUID>bb9b6556-5e97-4286-b600-7daf90a24b5b</UUID>
<Type>Natural</Type>
<Created UTC="14:13:4">2022.10.24</Created>
<LastModified UTC="9:35:21">2022.10.31</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  "                4U`Q(1G
eR@%;EsƴͲ1Zm'>}XVU,(q޵
n	h`Řhy/&6*UF-YFLi>82mpp(213oez:ZXeuT岛ymZ:疏%)        <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  	<?xml version="1.0"?><Features nextValidID="8"><Feature recentID="7" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="625-648" color="#ff9900" type="standard"/></Feature><Feature recentID="6" name="Terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="580-631" color="#ffffff" type="standard"/><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q><Q name="label"><V text="Terminator"/></Q></Feature><Feature recentID="5" name="NanoLuc" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="21-536" color="#a6acb3" type="standard"/><Q name="note"><V text="/Transferred_From=pD881-SR:145030 PCR Product"/><V text="/Transferred_Similarity=100.00%"/></Q><Q name="label"><V text="NanoLuc"/></Q><Q name="gene"><V text="NanoLuc"/></Q></Feature><Feature recentID="4" name="NL4" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="147-166" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL4"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="3" name="NL3" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="122-141" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL3"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="2" name="NL2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="92-111" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q><Q name="label"><V text="NL2"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="1" name="NL1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="67-86" color="#a6acb3" type="standard"/><Q name="note"><V text="/created_by=User"/></Q><Q name="label"><V text="NL1"/><V text="nonstandard type:  CRISPR"/></Q></Feature><Feature recentID="0" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="649-668" color="#a6acb3" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="8" name="1290_Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"><BindingSite location="624-667" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="624-667" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="624-667" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="624-667" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="7" name="1289_Nanoluc-F2" sequence="gcagtaaaggagaaaattttatggtttttactctggaagattttg"><BindingSite location="0-44" boundStrand="0" annealedBases="gcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="62"><Component hybridizedRange="0-44" bases="gcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-44" boundStrand="0" annealedBases="gcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="62"><Component hybridizedRange="0-44" bases="gcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="0" name="976_Nanoluc-R" sequence="ggaagataggcaattagtagaagc"><BindingSite location="624-647" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="624-647" bases="ggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="624-647" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="624-647" bases="ggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="1" name="Nanoluc-F2a" sequence="atggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="20-44" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="20-44" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="20-44" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="20-44" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="2" name="Nanoluc-Fa" sequence="caaaatagtgaggaggatatatatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="19-44" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatata"/><Component hybridizedRange="19-44" bases="tatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="19-44" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatata"/><Component hybridizedRange="19-44" bases="tatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="3" name="Nanoluc-F2" sequence="gcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-44" boundStrand="0" annealedBases="gcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="62"><Component hybridizedRange="0-44" bases="gcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-44" boundStrand="0" annealedBases="gcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="62"><Component hybridizedRange="0-44" bases="gcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="4" name="Nanoluc-F" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-44" boundStrand="0" annealedBases="gcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="62"><Component bases="ctcct"/><Component hybridizedRange="0-44" bases="gcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-44" boundStrand="0" annealedBases="gcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="62"><Component bases="ctcct"/><Component hybridizedRange="0-44" bases="gcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="5" name="Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="624-667" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="624-667" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="624-667" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="624-667" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="6" name="170_mini CTX1-R6K-F" sequence="CGTTCCACTGAGCGTCAGACC"><BindingSite location="55-64" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="55-64" bases="GCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="55-64" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="55-64" bases="GCGTCAGACC"/></BindingSite></Primer></Primers>
  )<Notes>
<UUID>bb9b6556-5e97-4286-b600-7daf90a24b5b</UUID>
<Type>Natural</Type>
<Created UTC="14:13:4">2022.10.24</Created>
<LastModified UTC="8:20:1">2022.10.31</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  %Aj*jh2jԾ N+~!23l/EVkboVձnK.JmBis"fjɅʨpQ|'`Q._O+̇\"
Laxގ]nU7l#F[Vg
ot        <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  	<?xml version="1.0"?><Features nextValidID="8"><Feature recentID="7" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="654-673" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="NL1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-91" color="#a6acb3" type="standard"/><Q name="label"><V text="NL1"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="5" name="NL2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="97-116" color="#a6acb3" type="standard"/><Q name="label"><V text="NL2"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q></Feature><Feature recentID="4" name="NL3" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-146" color="#a6acb3" type="standard"/><Q name="label"><V text="NL3"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="3" name="NL4" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="152-171" color="#a6acb3" type="standard"/><Q name="label"><V text="NL4"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="2" name="NanoLuc" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="26-541" color="#a6acb3" type="standard"/><Q name="gene"><V text="NanoLuc"/></Q><Q name="label"><V text="NanoLuc"/></Q><Q name="note"><V text="/Transferred_From=pD881-SR:145030 PCR Product"/><V text="/Transferred_Similarity=100.00%"/></Q></Feature><Feature recentID="1" name="Terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="585-636" color="#ffffff" type="standard"/><Q name="label"><V text="Terminator"/></Q><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q></Feature><Feature recentID="0" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="630-653" color="#ff9900" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="976_Nanoluc-R" sequence="ggaagataggcaattagtagaagc"><BindingSite location="629-652" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="629-652" bases="ggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="629-652" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="629-652" bases="ggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="6" name="1143_Clo-TetR-R" sequence="aaaattttctcctttactgcaggag"><BindingSite location="0-24" boundStrand="1" annealedBases="aaaattttctcctttactgcaggag" meltingTemperature="55"><Component hybridizedRange="0-24" bases="aaaattttctcctttactgcaggag"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="1" annealedBases="aaaattttctcctttactgcaggag" meltingTemperature="55"><Component hybridizedRange="0-24" bases="aaaattttctcctttactgcaggag"/></BindingSite></Primer><Primer recentID="5" name="Nanoluc-F2a" sequence="atggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="25-49" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="25-49" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="25-49" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="25-49" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="4" name="Nanoluc-Fa" sequence="caaaatagtgaggaggatatatatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="24-49" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatata"/><Component hybridizedRange="24-49" bases="tatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="24-49" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatata"/><Component hybridizedRange="24-49" bases="tatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="0" name="Nanoluc-F2" sequence="gcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="5-49" boundStrand="0" annealedBases="gcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="62"><Component hybridizedRange="5-49" bases="gcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="5-49" boundStrand="0" annealedBases="gcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="62"><Component hybridizedRange="5-49" bases="gcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="1" name="Nanoluc-F" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-49" boundStrand="0" annealedBases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="64"><Component hybridizedRange="0-49" bases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-49" boundStrand="0" annealedBases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="64"><Component hybridizedRange="0-49" bases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="2" name="Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="629-672" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="629-672" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="629-672" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="629-672" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="3" name="170_mini CTX1-R6K-F" sequence="CGTTCCACTGAGCGTCAGACC"><BindingSite location="60-69" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="60-69" bases="GCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="60-69" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="60-69" bases="GCGTCAGACC"/></BindingSite></Primer></Primers>
  *<Notes>
<UUID>bb9b6556-5e97-4286-b600-7daf90a24b5b</UUID>
<Type>Natural</Type>
<Created UTC="14:13:4">2022.10.24</Created>
<LastModified UTC="14:31:6">2022.10.24</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                  %Aj*jh2jԾ N+~!23l/EVkboVձnK.JmBis"fjɅʨpQ|'`Q._O+̇\"
Laxގ]nU7l#F[Vg
ot[        <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  	<?xml version="1.0"?><Features nextValidID="8"><Feature recentID="7" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="654-678" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="NL1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-91" color="#a6acb3" type="standard"/><Q name="label"><V text="NL1"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="5" name="NL2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="97-116" color="#a6acb3" type="standard"/><Q name="label"><V text="NL2"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q></Feature><Feature recentID="4" name="NL3" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-146" color="#a6acb3" type="standard"/><Q name="label"><V text="NL3"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="3" name="NL4" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="152-171" color="#a6acb3" type="standard"/><Q name="label"><V text="NL4"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="2" name="NanoLuc" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="26-541" color="#a6acb3" type="standard"/><Q name="gene"><V text="NanoLuc"/></Q><Q name="label"><V text="NanoLuc"/></Q><Q name="note"><V text="/Transferred_From=pD881-SR:145030 PCR Product"/><V text="/Transferred_Similarity=100.00%"/></Q></Feature><Feature recentID="1" name="Terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="585-636" color="#ffffff" type="standard"/><Q name="label"><V text="Terminator"/></Q><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q></Feature><Feature recentID="0" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="630-653" color="#ff9900" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="976_Nanoluc-R" sequence="ggaagataggcaattagtagaagc"><BindingSite location="629-652" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="629-652" bases="ggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="629-652" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component hybridizedRange="629-652" bases="ggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="6" name="1143_Clo-TetR-R" sequence="aaaattttctcctttactgcaggag"><BindingSite location="0-24" boundStrand="1" annealedBases="aaaattttctcctttactgcaggag" meltingTemperature="55"><Component hybridizedRange="0-24" bases="aaaattttctcctttactgcaggag"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="1" annealedBases="aaaattttctcctttactgcaggag" meltingTemperature="55"><Component hybridizedRange="0-24" bases="aaaattttctcctttactgcaggag"/></BindingSite></Primer><Primer recentID="5" name="Nanoluc-F2a" sequence="atggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="25-49" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="25-49" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="25-49" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="25-49" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="4" name="Nanoluc-Fa" sequence="caaaatagtgaggaggatatatatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="24-49" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatata"/><Component hybridizedRange="24-49" bases="tatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="24-49" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatata"/><Component hybridizedRange="24-49" bases="tatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="0" name="Nanoluc-F2" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-49" boundStrand="0" annealedBases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="64"><Component hybridizedRange="0-49" bases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-49" boundStrand="0" annealedBases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="64"><Component hybridizedRange="0-49" bases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="1" name="Nanoluc-F" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-49" boundStrand="0" annealedBases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="64"><Component hybridizedRange="0-49" bases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-49" boundStrand="0" annealedBases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="64"><Component hybridizedRange="0-49" bases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="2" name="Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="629-672" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="629-672" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="629-672" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="65"><Component hybridizedRange="629-672" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="3" name="170_mini CTX1-R6K-F" sequence="CGTTCCACTGAGCGTCAGACC"><BindingSite location="60-69" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="60-69" bases="GCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="60-69" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="60-69" bases="GCGTCAGACC"/></BindingSite></Primer></Primers>
  +<Notes>
<UUID>bb9b6556-5e97-4286-b600-7daf90a24b5b</UUID>
<Type>Natural</Type>
<Created UTC="14:13:4">2022.10.24</Created>
<LastModified UTC="14:29:32">2022.10.24</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  4                %Aj*jh2jԾ N+~!23l/EVkboVձnK.JmBis"fjɅʨpQ|'`Q._O+̇\"
Laxގ]nU7l#F[V        <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  	<?xml version="1.0"?><Features nextValidID="7"><Feature recentID="6" name="NL1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-91" color="#a6acb3" type="standard"/><Q name="label"><V text="NL1"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="5" name="NL2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="97-116" color="#a6acb3" type="standard"/><Q name="label"><V text="NL2"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q></Feature><Feature recentID="4" name="NL3" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-146" color="#a6acb3" type="standard"/><Q name="label"><V text="NL3"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="3" name="NL4" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="152-171" color="#a6acb3" type="standard"/><Q name="label"><V text="NL4"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="2" name="NanoLuc" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="26-541" color="#a6acb3" type="standard"/><Q name="gene"><V text="NanoLuc"/></Q><Q name="label"><V text="NanoLuc"/></Q><Q name="note"><V text="/Transferred_From=pD881-SR:145030 PCR Product"/><V text="/Transferred_Similarity=100.00%"/></Q></Feature><Feature recentID="1" name="Terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="585-636" color="#ffffff" type="standard"/><Q name="label"><V text="Terminator"/></Q><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q></Feature><Feature recentID="0" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="630-653" color="#ff9900" type="standard"/></Feature></Features>
  $<?xml version="1.0"?><Primers nextValidID="8"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="7" name="976_Nanoluc-R" sequence="ggaagataggcaattagtagaagc"><BindingSite location="629-652" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="55"><Component hybridizedRange="629-652" bases="ggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="629-652" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="55"><Component hybridizedRange="629-652" bases="ggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="6" name="1143_Clo-TetR-R" sequence="aaaattttctcctttactgcaggag"><BindingSite location="0-24" boundStrand="1" annealedBases="aaaattttctcctttactgcaggag" meltingTemperature="55"><Component hybridizedRange="0-24" bases="aaaattttctcctttactgcaggag"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="1" annealedBases="aaaattttctcctttactgcaggag" meltingTemperature="55"><Component hybridizedRange="0-24" bases="aaaattttctcctttactgcaggag"/></BindingSite></Primer><Primer recentID="5" name="Nanoluc-F2a" sequence="atggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="25-49" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="25-49" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="25-49" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="25-49" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="4" name="Nanoluc-Fa" sequence="caaaatagtgaggaggatatatatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="24-49" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatata"/><Component hybridizedRange="24-49" bases="tatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="24-49" boundStrand="0" annealedBases="tatggtttttactctggaagattttg" meltingTemperature="54"><Component bases="caaaatagtgaggaggatata"/><Component hybridizedRange="24-49" bases="tatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="0" name="Nanoluc-F2" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-49" boundStrand="0" annealedBases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="64"><Component hybridizedRange="0-49" bases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-49" boundStrand="0" annealedBases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="64"><Component hybridizedRange="0-49" bases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="1" name="Nanoluc-F" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-49" boundStrand="0" annealedBases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="64"><Component hybridizedRange="0-49" bases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-49" boundStrand="0" annealedBases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" meltingTemperature="64"><Component hybridizedRange="0-49" bases="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="2" name="Nanoluc-R2" sequence="CTGATTCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="629-652" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component bases="CTGATTCTGTGGATAACCGTATTAC"/><Component hybridizedRange="629-652" bases="ggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="629-652" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="54"><Component bases="CTGATTCTGTGGATAACCGTATTAC"/><Component hybridizedRange="629-652" bases="ggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="3" name="170_mini CTX1-R6K-F" sequence="CGTTCCACTGAGCGTCAGACC"><BindingSite location="60-69" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="60-69" bases="GCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="60-69" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="60-69" bases="GCGTCAGACC"/></BindingSite></Primer></Primers>
  +<Notes>
<UUID>bb9b6556-5e97-4286-b600-7daf90a24b5b</UUID>
<Type>Natural</Type>
<Created UTC="14:13:4">2022.10.24</Created>
<LastModified UTC="14:29:10">2022.10.24</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
          t        t`Q(1G
eR@%;EsƴͲ1Zm'>}XVU,(q޵
n	h`Řhy/&6*UF-YFLi>82mpp(213oez:ZXeuT岛ymZ:疏      s  <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  	<?xml version="1.0"?><Features nextValidID="7"><Feature recentID="6" name="NL1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="47-66" color="#a6acb3" type="standard"/><Q name="label"><V text="NL1"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="5" name="NL2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="72-91" color="#a6acb3" type="standard"/><Q name="label"><V text="NL2"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q></Feature><Feature recentID="4" name="NL3" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="102-121" color="#a6acb3" type="standard"/><Q name="label"><V text="NL3"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="3" name="NL4" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="127-146" color="#a6acb3" type="standard"/><Q name="label"><V text="NL4"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="2" name="NanoLuc" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-516" color="#a6acb3" type="standard"/><Q name="gene"><V text="NanoLuc"/></Q><Q name="label"><V text="NanoLuc"/></Q><Q name="note"><V text="/Transferred_From=pD881-SR:145030 PCR Product"/><V text="/Transferred_Similarity=100.00%"/></Q></Feature><Feature recentID="1" name="Terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="560-611" color="#ffffff" type="standard"/><Q name="label"><V text="Terminator"/></Q><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q></Feature><Feature recentID="0" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="605-628" color="#ff9900" type="standard"/></Feature></Features>
  	<?xml version="1.0"?><Primers nextValidID="4"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="Nanoluc-F2" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="ctcctgcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="ctcctgcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="1" name="Nanoluc-F" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="ctcctgcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="54"><Component bases="ctcctgcagtaaaggagaaaatttt"/><Component hybridizedRange="0-24" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="2" name="Nanoluc-R" sequence="ggaagataggcaattagtagaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="604-627" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="55"><Component hybridizedRange="604-627" bases="ggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="604-627" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="55"><Component hybridizedRange="604-627" bases="ggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="3" name="170_mini CTX1-R6K-F" sequence="CGTTCCACTGAGCGTCAGACC"><BindingSite location="35-44" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="35-44" bases="GCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="35-44" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="35-44" bases="GCGTCAGACC"/></BindingSite></Primer></Primers>
  *<Notes>
<UUID>bb9b6556-5e97-4286-b600-7daf90a24b5b</UUID>
<Type>Natural</Type>
<Created UTC="14:13:4">2022.10.24</Created>
<LastModified UTC="14:15:3">2022.10.24</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  W     
  ~        ~%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%ˡ/_QzlM=A1	#9EI&;TJ	;5u}L FUE sqW)@jFUAߑQJJԊRRH 5(UkuV f+i^D&UheVJTIy5"~a fՕPoch%|Jp`fF9f]"RVک4iHg5`W[dX=X]&_`[Pfj`fQF]VkRbj<%qhZY)zШ]IMU$Vp)U֩
JXD":{]Rެ%jhNUmyfYYefY{jY5WUF]V^uj]V^uj]V^uj]Vh"eeZA8QHYa%RvTjVRQiXiiXeUR*ZZFUfjzUijviueUhW[VVY^e]TkhҚ*F)zfJh+`JUE8ZTDjfEJdVZFek}4EWzUP1UDUejNefEI QUf)YVתxbVQJUiUJfiUf4IaYVVfyjETfUm^U(QQY'VymQ$UGiѦTYVERVF%EeiQFUQ+zVcRUfZYETUDqiUU5^VVU&YH:UDתnŕiiVm[Zٖ^*z^ꖮz֪k[:Rꥪ$ٷ{iUfZfeٹ%Y[z󯳷,8	P  /        5    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="30"><Feature recentID="29" name="H2-ori-pFN1ori" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3890-3906" color="#a6acb3" type="standard"/></Feature><Feature recentID="28" name="amplify from gBlock8" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1712-1733" color="#a6acb3" type="standard"/></Feature><Feature recentID="27" name="silent mutation" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2731-2731" color="#a6acb3" type="standard"/></Feature><Feature recentID="26" name="ACAGC" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1908-1912" color="#ffff00" type="standard"/></Feature><Feature recentID="25" name="Removed(ACAGC)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2729-2733" color="#c0c0c0" type="standard"/></Feature><Feature recentID="24" name="repA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2306-3529" color="#a6acb3" type="standard"/></Feature><Feature recentID="23" name="ori Fn" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1734-2279" color="#a6acb3" type="standard"/></Feature><Feature recentID="22" name="repA" directionality="1" translationMW="48741.57" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2306-3529" color="#ff9900" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="21" name="amplify from pUC19" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3890-3966" color="#a6acb3" type="standard"/></Feature><Feature recentID="20" name="amplify from pUC19" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-768" color="#a6acb3" type="standard"/></Feature><Feature recentID="19" name="Silent mutation" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1250-1250" color="#a6acb3" type="standard"/></Feature><Feature recentID="18" name="silent mutation" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1052-1052" color="#a6acb3" type="standard"/></Feature><Feature recentID="17" name="can be replaced by a terminator" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1700-1724" color="#a6acb3" type="standard"/></Feature><Feature recentID="16" name="CCNNNNNNNGG" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="244-254" color="#993366" type="standard"/></Feature><Feature recentID="15" name="CCNNNNNNNGG" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="523-533" color="#993366" type="standard"/></Feature><Feature recentID="14" name="Removed(CCNNNNNNNGG)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1249-1259" color="#993366" type="standard"/></Feature><Feature recentID="13" name="ACAGC" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="308-312" color="#ffff00" type="standard"/></Feature><Feature recentID="12" name="ACAGC" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="353-357" color="#ffff00" type="standard"/></Feature><Feature recentID="11" name="Removed(ACAGC)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1048-1051" color="#ffff00" type="standard"/></Feature><Feature recentID="10" name="Removed(CATG)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1053-1055" color="#0000ff" type="standard"/></Feature><Feature recentID="9" name="GATGC" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="132-136" color="#ff0000" type="standard"/></Feature><Feature recentID="8" name="CAT resistance to chloramphenicol" directionality="1" translationMW="24381.28" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="966-1589" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="105-693" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="6" name="putative RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="955-960" color="#a6acb3" type="standard"/></Feature><Feature recentID="5" name="Putative promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="816-965" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="putative terminator from pFP14" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1590-1699" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="Feature 23" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="546-546" color="#a6acb3" type="standard"/></Feature><Feature recentID="2" name="gBlock8" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="769-1733" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3947-3966" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-20" color="#a6acb3" type="standard"/></Feature></Features>
  7h<?xml version="1.0"?><Primers nextValidID="27"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="170_mini CTX1-R6K-F" sequence="CGTTCCACTGAGCGTCAGACC"><BindingSite location="718-738" boundStrand="1" annealedBases="CGTTCCACTGAGCGTCAGACC" meltingTemperature="63"><Component hybridizedRange="718-738" bases="CGTTCCACTGAGCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="718-738" boundStrand="1" annealedBases="CGTTCCACTGAGCGTCAGACC" meltingTemperature="63"><Component hybridizedRange="718-738" bases="CGTTCCACTGAGCGTCAGACC"/></BindingSite></Primer><Primer recentID="1" name="gBlock10-pFN1-F" sequence="GTTAGTAAAGTGTGAGAGCTTG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1711-1732" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="1711-1732" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite><BindingSite simplified="1" location="1711-1732" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="1711-1732" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite></Primer><Primer recentID="2" name="620_gBlock8-pCWU-catP-F" sequence="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC"><BindingSite location="745-791" boundStrand="0" annealedBases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC" meltingTemperature="66"><Component hybridizedRange="745-791" bases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC"/></BindingSite><BindingSite simplified="1" location="745-791" boundStrand="0" annealedBases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC" meltingTemperature="66"><Component hybridizedRange="745-791" bases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC"/></BindingSite></Primer><Primer recentID="3" name="617_pCWU-ori-R" sequence="CATGACCAAAATCCCTTAACGTG"><BindingSite location="745-767" boundStrand="1" annealedBases="CATGACCAAAATCCCTTAACGTG" meltingTemperature="57"><Component hybridizedRange="745-767" bases="CATGACCAAAATCCCTTAACGTG"/></BindingSite><BindingSite simplified="1" location="745-767" boundStrand="1" annealedBases="CATGACCAAAATCCCTTAACGTG" meltingTemperature="57"><Component hybridizedRange="745-767" bases="CATGACCAAAATCCCTTAACGTG"/></BindingSite></Primer><Primer recentID="4" name="273_seq-NCTX-2-F" sequence="CGTTAAGGGATTTTGGTCATG"><BindingSite location="747-767" boundStrand="0" annealedBases="CGTTAAGGGATTTTGGTCATG" meltingTemperature="53"><Component hybridizedRange="747-767" bases="CGTTAAGGGATTTTGGTCATG"/></BindingSite><BindingSite simplified="1" location="747-767" boundStrand="0" annealedBases="CGTTAAGGGATTTTGGTCATG" meltingTemperature="53"><Component hybridizedRange="747-767" bases="CGTTAAGGGATTTTGGTCATG"/></BindingSite></Primer><Primer recentID="5" name="192_R6K-TcR-R" sequence="TATCAGCTCACTCAAAGGCG"><BindingSite location="3946-3965" boundStrand="0" annealedBases="TATCAGCTCACTCAAAGGCG" meltingTemperature="55"><Component hybridizedRange="3946-3965" bases="TATCAGCTCACTCAAAGGCG"/></BindingSite><BindingSite simplified="1" location="3946-3965" boundStrand="0" annealedBases="TATCAGCTCACTCAAAGGCG" meltingTemperature="55"><Component hybridizedRange="3946-3965" bases="TATCAGCTCACTCAAAGGCG"/></BindingSite></Primer><Primer recentID="6" name="193_FRT-attP-F" sequence="CGCCTTTGAGTGAGCTGATA"><BindingSite location="3946-3965" boundStrand="1" annealedBases="CGCCTTTGAGTGAGCTGATA" meltingTemperature="54"><Component hybridizedRange="3946-3965" bases="CGCCTTTGAGTGAGCTGATA"/></BindingSite><BindingSite simplified="1" location="3946-3965" boundStrand="1" annealedBases="CGCCTTTGAGTGAGCTGATA" meltingTemperature="54"><Component hybridizedRange="3946-3965" bases="CGCCTTTGAGTGAGCTGATA"/></BindingSite></Primer><Primer recentID="7" name="172_CTX-R6K_seq1" sequence="GTTCCTGGCCTTTTGCTGGC"><BindingSite location="58-77" boundStrand="1" annealedBases="GTTCCTGGCCTTTTGCTGGC" meltingTemperature="61"><Component hybridizedRange="58-77" bases="GTTCCTGGCCTTTTGCTGGC"/></BindingSite><BindingSite simplified="1" location="58-77" boundStrand="1" annealedBases="GTTCCTGGCCTTTTGCTGGC" meltingTemperature="61"><Component hybridizedRange="58-77" bases="GTTCCTGGCCTTTTGCTGGC"/></BindingSite></Primer><Primer recentID="8" name="119_Check-pSGKP-R" sequence="ttaccgcctttgagtgagc"><BindingSite location="3951-3965" boundStrand="1" annealedBases="cgcctttgagtgagc" meltingTemperature="52"><Component bases="ttac"/><Component hybridizedRange="3951-3965" bases="cgcctttgagtgagc"/></BindingSite><BindingSite simplified="1" location="3951-3965" boundStrand="1" annealedBases="cgcctttgagtgagc" meltingTemperature="52"><Component bases="ttac"/><Component hybridizedRange="3951-3965" bases="cgcctttgagtgagc"/></BindingSite></Primer><Primer recentID="9" name="100_ F1-Tn7L-oriT-R" sequence="AACGCCAGCAACGCGGCC"><BindingSite location="86-103" boundStrand="1" annealedBases="AACGCCAGCAACGCGGCC" meltingTemperature="67"><Component hybridizedRange="86-103" bases="AACGCCAGCAACGCGGCC"/></BindingSite><BindingSite simplified="1" location="86-103" boundStrand="1" annealedBases="AACGCCAGCAACGCGGCC" meltingTemperature="67"><Component hybridizedRange="86-103" bases="AACGCCAGCAACGCGGCC"/></BindingSite></Primer><Primer recentID="10" name="616_pCWU-ori-F" sequence="CTTCCGCTTCCTCGCTC"><BindingSite location="3889-3905" boundStrand="0" annealedBases="CTTCCGCTTCCTCGCTC" meltingTemperature="57"><Component hybridizedRange="3889-3905" bases="CTTCCGCTTCCTCGCTC"/></BindingSite><BindingSite simplified="1" location="3889-3905" boundStrand="0" annealedBases="CTTCCGCTTCCTCGCTC" meltingTemperature="57"><Component hybridizedRange="3889-3905" bases="CTTCCGCTTCCTCGCTC"/></BindingSite></Primer><Primer recentID="11" name="594_Ori-R" sequence="gcgagcggtatcagctc"><BindingSite location="3938-3954" boundStrand="0" annealedBases="gcgagcggtatcagctc" meltingTemperature="57"><Component hybridizedRange="3938-3954" bases="gcgagcggtatcagctc"/></BindingSite><BindingSite simplified="1" location="3938-3954" boundStrand="0" annealedBases="gcgagcggtatcagctc" meltingTemperature="57"><Component hybridizedRange="3938-3954" bases="gcgagcggtatcagctc"/></BindingSite></Primer><Primer recentID="12" name="gBlock8-catP-pFN1-R" sequence="CAAGCTCTCACACTTTAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1715-1732" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="1715-1732" bases="CAAGCTCTCACACTTTAC"/></BindingSite><BindingSite simplified="1" location="1715-1732" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="1715-1732" bases="CAAGCTCTCACACTTTAC"/></BindingSite></Primer><Primer recentID="13" name="733_gBlock10-pFN1-R" sequence="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG"><BindingSite location="3868-3905" boundStrand="1" annealedBases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG" meltingTemperature="63"><Component hybridizedRange="3868-3905" bases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG"/></BindingSite><BindingSite simplified="1" location="3868-3905" boundStrand="1" annealedBases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG" meltingTemperature="63"><Component hybridizedRange="3868-3905" bases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG"/></BindingSite></Primer><Primer recentID="14" name="732_gBlock10-pFN1-F" sequence="GTTAGTAAAGTGTGAGAGCTTG"><BindingSite location="1711-1732" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="1711-1732" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite><BindingSite simplified="1" location="1711-1732" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="1711-1732" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite></Primer><Primer recentID="15" name="734_gBlock8-catP-pFN1-R" sequence="CAAGCTCTCACACTTTAC"><BindingSite location="1715-1732" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="1715-1732" bases="CAAGCTCTCACACTTTAC"/></BindingSite><BindingSite simplified="1" location="1715-1732" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="1715-1732" bases="CAAGCTCTCACACTTTAC"/></BindingSite></Primer><Primer recentID="16" name="907_LL-test-R" sequence="CTATGATACCGTGGTCAACCT"><BindingSite location="1380-1400" boundStrand="0" annealedBases="CTATGATACCGTGGTCAACCT" meltingTemperature="57"><Component hybridizedRange="1380-1400" bases="CTATGATACCGTGGTCAACCT"/></BindingSite><BindingSite simplified="1" location="1380-1400" boundStrand="0" annealedBases="CTATGATACCGTGGTCAACCT" meltingTemperature="57"><Component hybridizedRange="1380-1400" bases="CTATGATACCGTGGTCAACCT"/></BindingSite></Primer><Primer recentID="17" name="908_LL test-F" sequence="gctcacgctgtaggtatctc"><BindingSite location="301-320" boundStrand="0" annealedBases="gctcacgctgtaggtatctc" meltingTemperature="56"><Component hybridizedRange="301-320" bases="gctcacgctgtaggtatctc"/></BindingSite><BindingSite simplified="1" location="301-320" boundStrand="0" annealedBases="gctcacgctgtaggtatctc" meltingTemperature="56"><Component hybridizedRange="301-320" bases="gctcacgctgtaggtatctc"/></BindingSite></Primer><Primer recentID="18" name="pFN1-backbone-R" sequence="ctaagttccctctcaaattcaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="942-964" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="942-964" bases="ctaagttccctctcaaattcaag"/></BindingSite><BindingSite simplified="1" location="942-964" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="942-964" bases="ctaagttccctctcaaattcaag"/></BindingSite></Primer><Primer recentID="19" name="pFN1-backbone-F" sequence="cttcaggtttgtctgtaactaaaaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1589-1614" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="1589-1614" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite><BindingSite simplified="1" location="1589-1614" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="1589-1614" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite></Primer><Primer recentID="20" name="pFN1-catP-backbone-F" sequence="GTAATACGGTTATCCACAGAATCAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite></Primer><Primer recentID="21" name="953_pFN1-backbone-F" sequence="cttcaggtttgtctgtaactaaaaac"><BindingSite location="1589-1614" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="1589-1614" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite><BindingSite simplified="1" location="1589-1614" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="1589-1614" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite></Primer><Primer recentID="22" name="952_pFN1-backbone-R" sequence="ctaagttccctctcaaattcaag"><BindingSite location="942-964" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="942-964" bases="ctaagttccctctcaaattcaag"/></BindingSite><BindingSite simplified="1" location="942-964" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="942-964" bases="ctaagttccctctcaaattcaag"/></BindingSite></Primer><Primer recentID="23" name="1132_pFN1-catP-backbone-F" sequence="GTAATACGGTTATCCACAGAATCAG"><BindingSite location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite></Primer><Primer recentID="24" name="Overlap-nanoluc-R" sequence="CTGATTCTGTGGATAACCGTATTAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="54"><Component hybridizedRange="0-24" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="54"><Component hybridizedRange="0-24" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite></Primer><Primer recentID="25" name="977_pFN1-Nanoluc-F" sequence="gcttctactaattgcctatcttccGTAATACGGTTATCCACAGAATCAG"><BindingSite location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component bases="gcttctactaattgcctatcttcc"/><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component bases="gcttctactaattgcctatcttcc"/><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite></Primer><Primer recentID="26" name="1361_Overlap-nanoluc-R" sequence="CTGATTCTGTGGATAACCGTATTAC"><BindingSite location="0-24" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="54"><Component hybridizedRange="0-24" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="54"><Component hybridizedRange="0-24" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite></Primer></Primers>
  c<Notes>
<UUID>bda62445-771e-4ee2-85bb-5cde058880cc</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="10:59:56">2022.11.21</Created>
<LastModified UTC="10:59:56">2022.11.21</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  W     
  ~        ~%)m eE؄CUA|P(ʫuV;R1_ATgʿΟ~/>3NBnҰ+;8_+O3%ˡ/_QzlM=A1	#9EI&;TJ	;5u}L FUE sqW)@jFUAߑQJJԊRRH 5(UkuV f+i^D&UheVJTIy5"~a fՕPoch%|Jp`fF9f]"RVک4iHg5`W[dX=X]&_`[Pfj`fQF]VkRbj<%qhZY)zШ]IMU$Vp)U֩
JXD":{]Rެ%jhNUmyfYYefY{jY5WUF]V^uj]V^uj]V^uj]Vh"eeZA8QHYa%RvTjVRQiXiiXeUR*ZZFUfjzUijviueUhW[VVY^e]TkhҚ*F)zfJh+`JUE8ZTDjfEJdVZFek}4EWzUP1UDUejNefEI QUf)YVתxbVQJUiUJfiUf4IaYVVfyjETfUm^U(QQY'VymQ$UGiѦTYVERVF%EeiQFUQ+zVcRUfZYETUDqiUU5^VVU&YH:UDתnŕiiVm[Zٖ^*z^ꖮz֪k[:Rꥪ$ٷ{iUfZfeٹ%Y[z󯳷,8	P  /        5    <AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>Unmodified</UpstreamModification><DownstreamModification>Unmodified</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="30"><Feature recentID="29" name="H2-ori-pFN1ori" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3890-3906" color="#a6acb3" type="standard"/></Feature><Feature recentID="28" name="amplify from gBlock8" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1712-1733" color="#a6acb3" type="standard"/></Feature><Feature recentID="27" name="silent mutation" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2731-2731" color="#a6acb3" type="standard"/></Feature><Feature recentID="26" name="ACAGC" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1908-1912" color="#ffff00" type="standard"/></Feature><Feature recentID="25" name="Removed(ACAGC)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2729-2733" color="#c0c0c0" type="standard"/></Feature><Feature recentID="24" name="repA" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2306-3529" color="#a6acb3" type="standard"/></Feature><Feature recentID="23" name="ori Fn" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1734-2279" color="#a6acb3" type="standard"/></Feature><Feature recentID="22" name="repA" directionality="1" translationMW="48741.57" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2306-3529" color="#ff9900" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="21" name="amplify from pUC19" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3890-3966" color="#a6acb3" type="standard"/></Feature><Feature recentID="20" name="amplify from pUC19" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-768" color="#a6acb3" type="standard"/></Feature><Feature recentID="19" name="Silent mutation" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1250-1250" color="#a6acb3" type="standard"/></Feature><Feature recentID="18" name="silent mutation" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1052-1052" color="#a6acb3" type="standard"/></Feature><Feature recentID="17" name="can be replaced by a terminator" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1700-1724" color="#a6acb3" type="standard"/></Feature><Feature recentID="16" name="CCNNNNNNNGG" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="244-254" color="#993366" type="standard"/></Feature><Feature recentID="15" name="CCNNNNNNNGG" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="523-533" color="#993366" type="standard"/></Feature><Feature recentID="14" name="Removed(CCNNNNNNNGG)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1249-1259" color="#993366" type="standard"/></Feature><Feature recentID="13" name="ACAGC" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="308-312" color="#ffff00" type="standard"/></Feature><Feature recentID="12" name="ACAGC" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="353-357" color="#ffff00" type="standard"/></Feature><Feature recentID="11" name="Removed(ACAGC)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1048-1051" color="#ffff00" type="standard"/></Feature><Feature recentID="10" name="Removed(CATG)" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1053-1055" color="#0000ff" type="standard"/></Feature><Feature recentID="9" name="GATGC" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="132-136" color="#ff0000" type="standard"/></Feature><Feature recentID="8" name="CAT resistance to chloramphenicol" directionality="1" translationMW="24381.28" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="966-1589" color="#993366" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="105-693" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="6" name="putative RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="955-960" color="#a6acb3" type="standard"/></Feature><Feature recentID="5" name="Putative promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="816-965" color="#a6acb3" type="standard"/></Feature><Feature recentID="4" name="putative terminator from pFP14" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1590-1699" color="#ff0000" type="standard"/></Feature><Feature recentID="3" name="Feature 23" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="546-546" color="#a6acb3" type="standard"/></Feature><Feature recentID="2" name="gBlock8" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="769-1733" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3947-3966" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="H2" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-20" color="#a6acb3" type="standard"/></Feature></Features>
  7h<?xml version="1.0"?><Primers nextValidID="27"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="1361_Overlap-nanoluc-R" sequence="CTGATTCTGTGGATAACCGTATTAC"><BindingSite location="0-24" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="54"><Component hybridizedRange="0-24" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="54"><Component hybridizedRange="0-24" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite></Primer><Primer recentID="1" name="977_pFN1-Nanoluc-F" sequence="gcttctactaattgcctatcttccGTAATACGGTTATCCACAGAATCAG"><BindingSite location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component bases="gcttctactaattgcctatcttcc"/><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component bases="gcttctactaattgcctatcttcc"/><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite></Primer><Primer recentID="2" name="Overlap-nanoluc-R" sequence="CTGATTCTGTGGATAACCGTATTAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="54"><Component hybridizedRange="0-24" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="54"><Component hybridizedRange="0-24" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite></Primer><Primer recentID="3" name="1132_pFN1-catP-backbone-F" sequence="GTAATACGGTTATCCACAGAATCAG"><BindingSite location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite></Primer><Primer recentID="4" name="952_pFN1-backbone-R" sequence="ctaagttccctctcaaattcaag"><BindingSite location="942-964" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="942-964" bases="ctaagttccctctcaaattcaag"/></BindingSite><BindingSite simplified="1" location="942-964" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="942-964" bases="ctaagttccctctcaaattcaag"/></BindingSite></Primer><Primer recentID="5" name="953_pFN1-backbone-F" sequence="cttcaggtttgtctgtaactaaaaac"><BindingSite location="1589-1614" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="1589-1614" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite><BindingSite simplified="1" location="1589-1614" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="1589-1614" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite></Primer><Primer recentID="6" name="pFN1-catP-backbone-F" sequence="GTAATACGGTTATCCACAGAATCAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite><BindingSite simplified="1" location="0-24" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="0-24" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite></Primer><Primer recentID="7" name="pFN1-backbone-F" sequence="cttcaggtttgtctgtaactaaaaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1589-1614" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="1589-1614" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite><BindingSite simplified="1" location="1589-1614" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="1589-1614" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite></Primer><Primer recentID="8" name="pFN1-backbone-R" sequence="ctaagttccctctcaaattcaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="942-964" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="942-964" bases="ctaagttccctctcaaattcaag"/></BindingSite><BindingSite simplified="1" location="942-964" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="942-964" bases="ctaagttccctctcaaattcaag"/></BindingSite></Primer><Primer recentID="9" name="908_LL test-F" sequence="gctcacgctgtaggtatctc"><BindingSite location="301-320" boundStrand="0" annealedBases="gctcacgctgtaggtatctc" meltingTemperature="56"><Component hybridizedRange="301-320" bases="gctcacgctgtaggtatctc"/></BindingSite><BindingSite simplified="1" location="301-320" boundStrand="0" annealedBases="gctcacgctgtaggtatctc" meltingTemperature="56"><Component hybridizedRange="301-320" bases="gctcacgctgtaggtatctc"/></BindingSite></Primer><Primer recentID="10" name="907_LL-test-R" sequence="CTATGATACCGTGGTCAACCT"><BindingSite location="1380-1400" boundStrand="0" annealedBases="CTATGATACCGTGGTCAACCT" meltingTemperature="57"><Component hybridizedRange="1380-1400" bases="CTATGATACCGTGGTCAACCT"/></BindingSite><BindingSite simplified="1" location="1380-1400" boundStrand="0" annealedBases="CTATGATACCGTGGTCAACCT" meltingTemperature="57"><Component hybridizedRange="1380-1400" bases="CTATGATACCGTGGTCAACCT"/></BindingSite></Primer><Primer recentID="11" name="734_gBlock8-catP-pFN1-R" sequence="CAAGCTCTCACACTTTAC"><BindingSite location="1715-1732" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="1715-1732" bases="CAAGCTCTCACACTTTAC"/></BindingSite><BindingSite simplified="1" location="1715-1732" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="1715-1732" bases="CAAGCTCTCACACTTTAC"/></BindingSite></Primer><Primer recentID="12" name="732_gBlock10-pFN1-F" sequence="GTTAGTAAAGTGTGAGAGCTTG"><BindingSite location="1711-1732" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="1711-1732" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite><BindingSite simplified="1" location="1711-1732" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="1711-1732" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite></Primer><Primer recentID="13" name="733_gBlock10-pFN1-R" sequence="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG"><BindingSite location="3868-3905" boundStrand="1" annealedBases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG" meltingTemperature="63"><Component hybridizedRange="3868-3905" bases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG"/></BindingSite><BindingSite simplified="1" location="3868-3905" boundStrand="1" annealedBases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG" meltingTemperature="63"><Component hybridizedRange="3868-3905" bases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG"/></BindingSite></Primer><Primer recentID="14" name="gBlock8-catP-pFN1-R" sequence="CAAGCTCTCACACTTTAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1715-1732" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="1715-1732" bases="CAAGCTCTCACACTTTAC"/></BindingSite><BindingSite simplified="1" location="1715-1732" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="1715-1732" bases="CAAGCTCTCACACTTTAC"/></BindingSite></Primer><Primer recentID="15" name="594_Ori-R" sequence="gcgagcggtatcagctc"><BindingSite location="3938-3954" boundStrand="0" annealedBases="gcgagcggtatcagctc" meltingTemperature="57"><Component hybridizedRange="3938-3954" bases="gcgagcggtatcagctc"/></BindingSite><BindingSite simplified="1" location="3938-3954" boundStrand="0" annealedBases="gcgagcggtatcagctc" meltingTemperature="57"><Component hybridizedRange="3938-3954" bases="gcgagcggtatcagctc"/></BindingSite></Primer><Primer recentID="16" name="616_pCWU-ori-F" sequence="CTTCCGCTTCCTCGCTC"><BindingSite location="3889-3905" boundStrand="0" annealedBases="CTTCCGCTTCCTCGCTC" meltingTemperature="57"><Component hybridizedRange="3889-3905" bases="CTTCCGCTTCCTCGCTC"/></BindingSite><BindingSite simplified="1" location="3889-3905" boundStrand="0" annealedBases="CTTCCGCTTCCTCGCTC" meltingTemperature="57"><Component hybridizedRange="3889-3905" bases="CTTCCGCTTCCTCGCTC"/></BindingSite></Primer><Primer recentID="17" name="100_ F1-Tn7L-oriT-R" sequence="AACGCCAGCAACGCGGCC"><BindingSite location="86-103" boundStrand="1" annealedBases="AACGCCAGCAACGCGGCC" meltingTemperature="67"><Component hybridizedRange="86-103" bases="AACGCCAGCAACGCGGCC"/></BindingSite><BindingSite simplified="1" location="86-103" boundStrand="1" annealedBases="AACGCCAGCAACGCGGCC" meltingTemperature="67"><Component hybridizedRange="86-103" bases="AACGCCAGCAACGCGGCC"/></BindingSite></Primer><Primer recentID="18" name="119_Check-pSGKP-R" sequence="ttaccgcctttgagtgagc"><BindingSite location="3951-3965" boundStrand="1" annealedBases="cgcctttgagtgagc" meltingTemperature="52"><Component bases="ttac"/><Component hybridizedRange="3951-3965" bases="cgcctttgagtgagc"/></BindingSite><BindingSite simplified="1" location="3951-3965" boundStrand="1" annealedBases="cgcctttgagtgagc" meltingTemperature="52"><Component bases="ttac"/><Component hybridizedRange="3951-3965" bases="cgcctttgagtgagc"/></BindingSite></Primer><Primer recentID="19" name="172_CTX-R6K_seq1" sequence="GTTCCTGGCCTTTTGCTGGC"><BindingSite location="58-77" boundStrand="1" annealedBases="GTTCCTGGCCTTTTGCTGGC" meltingTemperature="61"><Component hybridizedRange="58-77" bases="GTTCCTGGCCTTTTGCTGGC"/></BindingSite><BindingSite simplified="1" location="58-77" boundStrand="1" annealedBases="GTTCCTGGCCTTTTGCTGGC" meltingTemperature="61"><Component hybridizedRange="58-77" bases="GTTCCTGGCCTTTTGCTGGC"/></BindingSite></Primer><Primer recentID="20" name="193_FRT-attP-F" sequence="CGCCTTTGAGTGAGCTGATA"><BindingSite location="3946-3965" boundStrand="1" annealedBases="CGCCTTTGAGTGAGCTGATA" meltingTemperature="54"><Component hybridizedRange="3946-3965" bases="CGCCTTTGAGTGAGCTGATA"/></BindingSite><BindingSite simplified="1" location="3946-3965" boundStrand="1" annealedBases="CGCCTTTGAGTGAGCTGATA" meltingTemperature="54"><Component hybridizedRange="3946-3965" bases="CGCCTTTGAGTGAGCTGATA"/></BindingSite></Primer><Primer recentID="21" name="192_R6K-TcR-R" sequence="TATCAGCTCACTCAAAGGCG"><BindingSite location="3946-3965" boundStrand="0" annealedBases="TATCAGCTCACTCAAAGGCG" meltingTemperature="55"><Component hybridizedRange="3946-3965" bases="TATCAGCTCACTCAAAGGCG"/></BindingSite><BindingSite simplified="1" location="3946-3965" boundStrand="0" annealedBases="TATCAGCTCACTCAAAGGCG" meltingTemperature="55"><Component hybridizedRange="3946-3965" bases="TATCAGCTCACTCAAAGGCG"/></BindingSite></Primer><Primer recentID="22" name="273_seq-NCTX-2-F" sequence="CGTTAAGGGATTTTGGTCATG"><BindingSite location="747-767" boundStrand="0" annealedBases="CGTTAAGGGATTTTGGTCATG" meltingTemperature="53"><Component hybridizedRange="747-767" bases="CGTTAAGGGATTTTGGTCATG"/></BindingSite><BindingSite simplified="1" location="747-767" boundStrand="0" annealedBases="CGTTAAGGGATTTTGGTCATG" meltingTemperature="53"><Component hybridizedRange="747-767" bases="CGTTAAGGGATTTTGGTCATG"/></BindingSite></Primer><Primer recentID="23" name="617_pCWU-ori-R" sequence="CATGACCAAAATCCCTTAACGTG"><BindingSite location="745-767" boundStrand="1" annealedBases="CATGACCAAAATCCCTTAACGTG" meltingTemperature="57"><Component hybridizedRange="745-767" bases="CATGACCAAAATCCCTTAACGTG"/></BindingSite><BindingSite simplified="1" location="745-767" boundStrand="1" annealedBases="CATGACCAAAATCCCTTAACGTG" meltingTemperature="57"><Component hybridizedRange="745-767" bases="CATGACCAAAATCCCTTAACGTG"/></BindingSite></Primer><Primer recentID="24" name="620_gBlock8-pCWU-catP-F" sequence="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC"><BindingSite location="745-791" boundStrand="0" annealedBases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC" meltingTemperature="66"><Component hybridizedRange="745-791" bases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC"/></BindingSite><BindingSite simplified="1" location="745-791" boundStrand="0" annealedBases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC" meltingTemperature="66"><Component hybridizedRange="745-791" bases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC"/></BindingSite></Primer><Primer recentID="25" name="gBlock10-pFN1-F" sequence="GTTAGTAAAGTGTGAGAGCTTG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="1711-1732" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="1711-1732" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite><BindingSite simplified="1" location="1711-1732" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="1711-1732" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite></Primer><Primer recentID="26" name="170_mini CTX1-R6K-F" sequence="CGTTCCACTGAGCGTCAGACC"><BindingSite location="718-738" boundStrand="1" annealedBases="CGTTCCACTGAGCGTCAGACC" meltingTemperature="63"><Component hybridizedRange="718-738" bases="CGTTCCACTGAGCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="718-738" boundStrand="1" annealedBases="CGTTCCACTGAGCGTCAGACC" meltingTemperature="63"><Component hybridizedRange="718-738" bases="CGTTCCACTGAGCGTCAGACC"/></BindingSite></Primer></Primers>
  ,<Notes>
<UUID>9e3418ad-ddb2-4dee-a64d-619798bae770</UUID>
<Type>Natural</Type>
<Created UTC="10:36:23">2022.11.21</Created>
<LastModified UTC="10:59:50">2022.11.21</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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j~ ٝD..vT}4\&FR	Z]p;i5\nð	},R.%e¤#F}˖.fs5,ٰ`Ꞔ΃F@#eMaE`Kz!|$A+ŚIլG  k^  ӫgg    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="15"><Feature recentID="0" name="ori Fn" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2662-3207" color="#a6acb3" type="standard"/></Feature><Feature recentID="1" name="repA" directionality="1" translationMW="48740.63" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3234-4457" color="#ff9900" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MDFSSIKKSLGLINFRDLKKYILGLHQKLGNLHITNITNKKIETIFLFEKFINDLDNNTLTIRVTKDSLYFFNIANSYLRFLFSDVRKLSGKYSKLLVPYLMEFSHKKEAEFEKERFFNILEVEESYRNNLSDFNKRILKPAVEELKTLFENLKVERLKNGRVIKGYKFSWTNDFNFQNKKDNIEEAEVVEEKENIASGELEKYFKSTFTDVNYSKKHKEVLEKLLKNNSLEYIKKYLSEQWEYVQNDKNILNKSAYFSKLILEEKAVYKNHLPADYEELKVEERNRNIESTNTITSLKDLVEKDITDYEVRKNITPEQIEQEVLFKIDVTEEEYNKIKEDWIIKRKDEVPNSDPKLLEIIFNASQSKKYNIINTKEEVNEKEKELHELEENIKRMQEELNKLKKEV*"/></Q></Feature><Feature recentID="2" name="CAT resistance to chloramphenicol" directionality="1" translationMW="24380.80" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1894-2517" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MVFEKIDKNSWNRKEYFDHYFASVPCTYSMTVKVDITQIKEKGMKLYPAMLYYIAMIVNRHSEFRTAINQDGELGIYDEMIPSYTIFHNDTETFSSLWTECKSDFKSFLADYESDTQRYGNNHRMEGKPNAPENIFNVSMIPWSTFDGFNLNLQKGYDYLIPIFTMGKYYKEDNKIILPLAIQVHHAVCDGFHICRFVNELQELINS*"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1033-1621" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="putative RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1883-1888" color="#a6acb3" type="standard"/></Feature><Feature recentID="5" name="Putative promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1744-1893" color="#a6acb3" type="standard"/></Feature><Feature recentID="6" name="putative terminator from pFP14" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2518-2627" color="#ff0000" type="standard"/></Feature><Feature recentID="7" name="Terminator" type="terminator" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="860-911" color="#ffffff" type="standard"/><Q name="label"><V text="Terminator"/></Q><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q></Feature><Feature recentID="8" name="NanoLuc" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="301-816" color="#a6acb3" type="standard"/><Q name="gene"><V text="NanoLuc"/></Q><Q name="label"><V text="NanoLuc"/></Q><Q name="note"><V text="/Transferred_From=pD881-SR:145030 PCR Product"/><V text="/Transferred_Similarity=100.00%"/></Q></Feature><Feature recentID="9" name="NL4" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="427-446" color="#a6acb3" type="standard"/><Q name="label"><V text="NL4"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="10" name="NL3" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="402-421" color="#a6acb3" type="standard"/><Q name="label"><V text="NL3"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="11" name="NL2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="372-391" color="#a6acb3" type="standard"/><Q name="label"><V text="NL2"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/><V text="/modified_by=User"/></Q></Feature><Feature recentID="12" name="NL1" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="347-366" color="#a6acb3" type="standard"/><Q name="label"><V text="NL1"/><V text="nonstandard type:  CRISPR"/></Q><Q name="note"><V text="/created_by=User"/></Q></Feature><Feature recentID="13" name="RBS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="287-295" color="#a6acb3" type="standard"/></Feature><Feature recentID="14" name="putative promoter" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="125-174" color="#a6acb3" type="standard"/></Feature></Features>
  f_<?xml version="1.0"?><Primers nextValidID="40"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="977_pFN1-Nanoluc-F" sequence="gcttctactaattgcctatcttccGTAATACGGTTATCCACAGAATCAG"><BindingSite location="904-952" boundStrand="0" annealedBases="gcttctactaattgcctatcttccGTAATACGGTTATCCACAGAATCAG" meltingTemperature="67"><Component hybridizedRange="904-952" bases="gcttctactaattgcctatcttccGTAATACGGTTATCCACAGAATCAG"/></BindingSite><BindingSite simplified="1" location="904-952" boundStrand="0" annealedBases="gcttctactaattgcctatcttccGTAATACGGTTATCCACAGAATCAG" meltingTemperature="67"><Component hybridizedRange="904-952" bases="gcttctactaattgcctatcttccGTAATACGGTTATCCACAGAATCAG"/></BindingSite></Primer><Primer recentID="1" name="Overlap-nanoluc-R" sequence="CTGATTCTGTGGATAACCGTATTAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="928-952" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="55"><Component hybridizedRange="928-952" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite><BindingSite simplified="1" location="928-952" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="55"><Component hybridizedRange="928-952" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite></Primer><Primer recentID="2" name="1132_pFN1-catP-backbone-F" sequence="GTAATACGGTTATCCACAGAATCAG"><BindingSite location="928-952" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="928-952" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite><BindingSite simplified="1" location="928-952" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="928-952" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite></Primer><Primer recentID="3" name="952_pFN1-backbone-R" sequence="ctaagttccctctcaaattcaag"><BindingSite location="54-76" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="53"><Component hybridizedRange="54-76" bases="ctaagttccctctcaaattcaag"/></BindingSite><BindingSite location="1870-1892" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="1870-1892" bases="ctaagttccctctcaaattcaag"/></BindingSite><BindingSite simplified="1" location="54-76" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="53"><Component hybridizedRange="54-76" bases="ctaagttccctctcaaattcaag"/></BindingSite><BindingSite simplified="1" location="1870-1892" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="1870-1892" bases="ctaagttccctctcaaattcaag"/></BindingSite></Primer><Primer recentID="4" name="953_pFN1-backbone-F" sequence="cttcaggtttgtctgtaactaaaaac"><BindingSite location="2517-2542" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="2517-2542" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite><BindingSite location="2577-2588" boundStrand="1" annealedBases="gtaacaaaaac" meltingTemperature="21"><Component bases="cttcaggtttgtct"/><Component hybridizedRange="2584-2588" bases="gtaac"/><Component bases="t"/><Component hybridizedRange="2577-2582" bases="aaaaac"/></BindingSite><BindingSite simplified="1" location="2517-2542" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="2517-2542" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite><BindingSite simplified="1" location="2577-2588" boundStrand="1" annealedBases="gtaacaaaaac" meltingTemperature="21"><Component bases="cttcaggtttgtct"/><Component hybridizedRange="2584-2588" bases="gtaac"/><Component bases="t"/><Component hybridizedRange="2577-2582" bases="aaaaac"/></BindingSite></Primer><Primer recentID="5" name="pFN1-catP-backbone-F" sequence="GTAATACGGTTATCCACAGAATCAG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="928-952" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="928-952" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite><BindingSite simplified="1" location="928-952" boundStrand="0" annealedBases="GTAATACGGTTATCCACAGAATCAG" meltingTemperature="55"><Component hybridizedRange="928-952" bases="GTAATACGGTTATCCACAGAATCAG"/></BindingSite></Primer><Primer recentID="6" name="pFN1-backbone-F" sequence="cttcaggtttgtctgtaactaaaaac" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2517-2542" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="2517-2542" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite><BindingSite location="2577-2588" boundStrand="1" annealedBases="gtaacaaaaac" meltingTemperature="21"><Component bases="cttcaggtttgtct"/><Component hybridizedRange="2584-2588" bases="gtaac"/><Component bases="t"/><Component hybridizedRange="2577-2582" bases="aaaaac"/></BindingSite><BindingSite simplified="1" location="2517-2542" boundStrand="0" annealedBases="cttcaggtttgtctgtaactaaaaac" meltingTemperature="54"><Component hybridizedRange="2517-2542" bases="cttcaggtttgtctgtaactaaaaac"/></BindingSite><BindingSite simplified="1" location="2577-2588" boundStrand="1" annealedBases="gtaacaaaaac" meltingTemperature="21"><Component bases="cttcaggtttgtct"/><Component hybridizedRange="2584-2588" bases="gtaac"/><Component bases="t"/><Component hybridizedRange="2577-2582" bases="aaaaac"/></BindingSite></Primer><Primer recentID="7" name="pFN1-backbone-R" sequence="ctaagttccctctcaaattcaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="54-76" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="53"><Component hybridizedRange="54-76" bases="ctaagttccctctcaaattcaag"/></BindingSite><BindingSite location="1870-1892" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="1870-1892" bases="ctaagttccctctcaaattcaag"/></BindingSite><BindingSite simplified="1" location="54-76" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="53"><Component hybridizedRange="54-76" bases="ctaagttccctctcaaattcaag"/></BindingSite><BindingSite simplified="1" location="1870-1892" boundStrand="1" annealedBases="ctaagttccctctcaaattcaag" meltingTemperature="54"><Component hybridizedRange="1870-1892" bases="ctaagttccctctcaaattcaag"/></BindingSite></Primer><Primer recentID="8" name="908_LL test-F" sequence="gctcacgctgtaggtatctc"><BindingSite location="1229-1248" boundStrand="0" annealedBases="gctcacgctgtaggtatctc" meltingTemperature="56"><Component hybridizedRange="1229-1248" bases="gctcacgctgtaggtatctc"/></BindingSite><BindingSite simplified="1" location="1229-1248" boundStrand="0" annealedBases="gctcacgctgtaggtatctc" meltingTemperature="56"><Component hybridizedRange="1229-1248" bases="gctcacgctgtaggtatctc"/></BindingSite></Primer><Primer recentID="9" name="907_LL-test-R" sequence="CTATGATACCGTGGTCAACCT"><BindingSite location="2308-2328" boundStrand="0" annealedBases="CTATGATACCGTGGTCAACCT" meltingTemperature="57"><Component hybridizedRange="2308-2328" bases="CTATGATACCGTGGTCAACCT"/></BindingSite><BindingSite simplified="1" location="2308-2328" boundStrand="0" annealedBases="CTATGATACCGTGGTCAACCT" meltingTemperature="57"><Component hybridizedRange="2308-2328" bases="CTATGATACCGTGGTCAACCT"/></BindingSite></Primer><Primer recentID="10" name="734_gBlock8-catP-pFN1-R" sequence="CAAGCTCTCACACTTTAC"><BindingSite location="2643-2660" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="2643-2660" bases="CAAGCTCTCACACTTTAC"/></BindingSite><BindingSite simplified="1" location="2643-2660" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="2643-2660" bases="CAAGCTCTCACACTTTAC"/></BindingSite></Primer><Primer recentID="11" name="732_gBlock10-pFN1-F" sequence="GTTAGTAAAGTGTGAGAGCTTG"><BindingSite location="2639-2660" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="2639-2660" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite><BindingSite simplified="1" location="2639-2660" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="2639-2660" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite></Primer><Primer recentID="12" name="733_gBlock10-pFN1-R" sequence="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG"><BindingSite location="4796-4833" boundStrand="1" annealedBases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG" meltingTemperature="63"><Component hybridizedRange="4796-4833" bases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG"/></BindingSite><BindingSite simplified="1" location="4796-4833" boundStrand="1" annealedBases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG" meltingTemperature="63"><Component hybridizedRange="4796-4833" bases="GAGCGAGGAAGCGGAAGTGATTTATTTTACTAGATATG"/></BindingSite></Primer><Primer recentID="13" name="gBlock8-catP-pFN1-R" sequence="CAAGCTCTCACACTTTAC" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2643-2660" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="2643-2660" bases="CAAGCTCTCACACTTTAC"/></BindingSite><BindingSite simplified="1" location="2643-2660" boundStrand="1" annealedBases="CAAGCTCTCACACTTTAC" meltingTemperature="51"><Component hybridizedRange="2643-2660" bases="CAAGCTCTCACACTTTAC"/></BindingSite></Primer><Primer recentID="14" name="594_Ori-R" sequence="gcgagcggtatcagctc"><BindingSite location="2127-2137" boundStrand="1" annealedBases="ggtatcactc" meltingTemperature="25"><Component bases="gcgagc"/><Component hybridizedRange="2131-2137" bases="ggtatca"/><Component bases="g"/><Component hybridizedRange="2127-2129" bases="ctc"/></BindingSite><BindingSite location="4866-8" boundStrand="0" annealedBases="gcgagcggtatcagctc" meltingTemperature="57"><Component hybridizedRange="4866-8" bases="gcgagcggtatcagctc"/></BindingSite><BindingSite simplified="1" location="2127-2137" boundStrand="1" annealedBases="ggtatcactc" meltingTemperature="25"><Component bases="gcgagc"/><Component hybridizedRange="2131-2137" bases="ggtatca"/><Component bases="g"/><Component hybridizedRange="2127-2129" bases="ctc"/></BindingSite><BindingSite simplified="1" location="4866-8" boundStrand="0" annealedBases="gcgagcggtatcagctc" meltingTemperature="57"><Component hybridizedRange="4866-8" bases="gcgagcggtatcagctc"/></BindingSite></Primer><Primer recentID="15" name="616_pCWU-ori-F" sequence="CTTCCGCTTCCTCGCTC"><BindingSite location="4817-4833" boundStrand="0" annealedBases="CTTCCGCTTCCTCGCTC" meltingTemperature="57"><Component hybridizedRange="4817-4833" bases="CTTCCGCTTCCTCGCTC"/></BindingSite><BindingSite simplified="1" location="4817-4833" boundStrand="0" annealedBases="CTTCCGCTTCCTCGCTC" meltingTemperature="57"><Component hybridizedRange="4817-4833" bases="CTTCCGCTTCCTCGCTC"/></BindingSite></Primer><Primer recentID="16" name="100_ F1-Tn7L-oriT-R" sequence="AACGCCAGCAACGCGGCC"><BindingSite location="1014-1031" boundStrand="1" annealedBases="AACGCCAGCAACGCGGCC" meltingTemperature="67"><Component hybridizedRange="1014-1031" bases="AACGCCAGCAACGCGGCC"/></BindingSite><BindingSite simplified="1" location="1014-1031" boundStrand="1" annealedBases="AACGCCAGCAACGCGGCC" meltingTemperature="67"><Component hybridizedRange="1014-1031" bases="AACGCCAGCAACGCGGCC"/></BindingSite></Primer><Primer recentID="17" name="119_Check-pSGKP-R" sequence="ttaccgcctttgagtgagc"><BindingSite location="5-21" boundStrand="1" annealedBases="accgcctttgagtgagc" meltingTemperature="57"><Component bases="tt"/><Component hybridizedRange="5-21" bases="accgcctttgagtgagc"/></BindingSite><BindingSite simplified="1" location="5-21" boundStrand="1" annealedBases="accgcctttgagtgagc" meltingTemperature="57"><Component bases="tt"/><Component hybridizedRange="5-21" bases="accgcctttgagtgagc"/></BindingSite></Primer><Primer recentID="18" name="172_CTX-R6K_seq1" sequence="GTTCCTGGCCTTTTGCTGGC"><BindingSite location="320-329" boundStrand="0" annealedBases="TTTTGTGGC" meltingTemperature="10"><Component bases="GTTCCTGGCC"/><Component hybridizedRange="320-324" bases="TTTTG"/><Component bases="C"/><Component hybridizedRange="326-329" bases="TGGC"/></BindingSite><BindingSite location="986-1005" boundStrand="1" annealedBases="GTTCCTGGCCTTTTGCTGGC" meltingTemperature="61"><Component hybridizedRange="986-1005" bases="GTTCCTGGCCTTTTGCTGGC"/></BindingSite><BindingSite simplified="1" location="320-329" boundStrand="0" annealedBases="TTTTGTGGC" meltingTemperature="10"><Component bases="GTTCCTGGCC"/><Component hybridizedRange="320-324" bases="TTTTG"/><Component bases="C"/><Component hybridizedRange="326-329" bases="TGGC"/></BindingSite><BindingSite simplified="1" location="986-1005" boundStrand="1" annealedBases="GTTCCTGGCCTTTTGCTGGC" meltingTemperature="61"><Component hybridizedRange="986-1005" bases="GTTCCTGGCCTTTTGCTGGC"/></BindingSite></Primer><Primer recentID="19" name="193_FRT-attP-F" sequence="CGCCTTTGAGTGAGCTGATA"><BindingSite location="0-19" boundStrand="1" annealedBases="CGCCTTTGAGTGAGCTGATA" meltingTemperature="54"><Component hybridizedRange="0-19" bases="CGCCTTTGAGTGAGCTGATA"/></BindingSite><BindingSite simplified="1" location="0-19" boundStrand="1" annealedBases="CGCCTTTGAGTGAGCTGATA" meltingTemperature="54"><Component hybridizedRange="0-19" bases="CGCCTTTGAGTGAGCTGATA"/></BindingSite></Primer><Primer recentID="20" name="192_R6K-TcR-R" sequence="TATCAGCTCACTCAAAGGCG"><BindingSite location="0-19" boundStrand="0" annealedBases="TATCAGCTCACTCAAAGGCG" meltingTemperature="57"><Component hybridizedRange="0-19" bases="TATCAGCTCACTCAAAGGCG"/></BindingSite><BindingSite simplified="1" location="0-19" boundStrand="0" annealedBases="TATCAGCTCACTCAAAGGCG" meltingTemperature="57"><Component hybridizedRange="0-19" bases="TATCAGCTCACTCAAAGGCG"/></BindingSite></Primer><Primer recentID="21" name="273_seq-NCTX-2-F" sequence="CGTTAAGGGATTTTGGTCATG"><BindingSite location="1675-1695" boundStrand="0" annealedBases="CGTTAAGGGATTTTGGTCATG" meltingTemperature="53"><Component hybridizedRange="1675-1695" bases="CGTTAAGGGATTTTGGTCATG"/></BindingSite><BindingSite simplified="1" location="1675-1695" boundStrand="0" annealedBases="CGTTAAGGGATTTTGGTCATG" meltingTemperature="53"><Component hybridizedRange="1675-1695" bases="CGTTAAGGGATTTTGGTCATG"/></BindingSite></Primer><Primer recentID="22" name="617_pCWU-ori-R" sequence="CATGACCAAAATCCCTTAACGTG"><BindingSite location="1673-1695" boundStrand="1" annealedBases="CATGACCAAAATCCCTTAACGTG" meltingTemperature="57"><Component hybridizedRange="1673-1695" bases="CATGACCAAAATCCCTTAACGTG"/></BindingSite><BindingSite simplified="1" location="1673-1695" boundStrand="1" annealedBases="CATGACCAAAATCCCTTAACGTG" meltingTemperature="57"><Component hybridizedRange="1673-1695" bases="CATGACCAAAATCCCTTAACGTG"/></BindingSite></Primer><Primer recentID="23" name="620_gBlock8-pCWU-catP-F" sequence="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC"><BindingSite location="1673-1719" boundStrand="0" annealedBases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC" meltingTemperature="66"><Component hybridizedRange="1673-1719" bases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC"/></BindingSite><BindingSite simplified="1" location="1673-1719" boundStrand="0" annealedBases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC" meltingTemperature="66"><Component hybridizedRange="1673-1719" bases="CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC"/></BindingSite></Primer><Primer recentID="24" name="gBlock10-pFN1-F" sequence="GTTAGTAAAGTGTGAGAGCTTG" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="2639-2660" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="2639-2660" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite><BindingSite simplified="1" location="2639-2660" boundStrand="0" annealedBases="GTTAGTAAAGTGTGAGAGCTTG" meltingTemperature="53"><Component hybridizedRange="2639-2660" bases="GTTAGTAAAGTGTGAGAGCTTG"/></BindingSite></Primer><Primer recentID="25" name="975_Nanoluc-F" sequence="caaaatagtgaggaggatatatatggtttttactctggaagattttg"><BindingSite location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="26" name="1361_Overlap-nanoluc-R" sequence="CTGATTCTGTGGATAACCGTATTAC"><BindingSite location="928-952" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="55"><Component hybridizedRange="928-952" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite><BindingSite simplified="1" location="928-952" boundStrand="1" annealedBases="CTGATTCTGTGGATAACCGTATTAC" meltingTemperature="55"><Component hybridizedRange="928-952" bases="CTGATTCTGTGGATAACCGTATTAC"/></BindingSite></Primer><Primer recentID="27" name="1360_Nanoluc-F2a" sequence="atggtttttactctggaagattttg"><BindingSite location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="28" name="1399_4.5sRNA-backbone-F" sequence="tgaactgcacttgctttgat"><BindingSite location="816-835" boundStrand="0" annealedBases="tgaactgcacttgctttgat" meltingTemperature="55"><Component hybridizedRange="816-835" bases="tgaactgcacttgctttgat"/></BindingSite><BindingSite simplified="1" location="816-835" boundStrand="0" annealedBases="tgaactgcacttgctttgat" meltingTemperature="55"><Component hybridizedRange="816-835" bases="tgaactgcacttgctttgat"/></BindingSite></Primer><Primer recentID="29" name="1290_Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"><BindingSite location="904-947" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="66"><Component hybridizedRange="904-947" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="904-947" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="66"><Component hybridizedRange="904-947" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="30" name="1289_Nanoluc-F2" sequence="gcagtaaaggagaaaattttatggtttttactctggaagattttg"><BindingSite location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="31" name="976_Nanoluc-R" sequence="ggaagataggcaattagtagaagc"><BindingSite location="904-927" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="55"><Component hybridizedRange="904-927" bases="ggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="904-927" boundStrand="1" annealedBases="ggaagataggcaattagtagaagc" meltingTemperature="55"><Component hybridizedRange="904-927" bases="ggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="32" name="Nanoluc-F2a" sequence="atggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="33" name="Nanoluc-Fa" sequence="caaaatagtgaggaggatatatatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component bases="caaaatagtgaggaggatatat"/><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="34" name="Nanoluc-F2" sequence="gcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component bases="gcagtaaaggagaaaatttt"/><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="35" name="Nanoluc-F" sequence="ctcctgcagtaaaggagaaaattttatggtttttactctggaagattttg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component bases="ctcctgcagtaaaggagaaaatttt"/><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite><BindingSite simplified="1" location="300-324" boundStrand="0" annealedBases="atggtttttactctggaagattttg" meltingTemperature="55"><Component bases="ctcctgcagtaaaggagaaaatttt"/><Component hybridizedRange="300-324" bases="atggtttttactctggaagattttg"/></BindingSite></Primer><Primer recentID="36" name="Nanoluc-R2" sequence="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="904-947" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="66"><Component hybridizedRange="904-947" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite><BindingSite simplified="1" location="904-947" boundStrand="1" annealedBases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc" meltingTemperature="66"><Component hybridizedRange="904-947" bases="TCTGTGGATAACCGTATTACggaagataggcaattagtagaagc"/></BindingSite></Primer><Primer recentID="37" name="170_mini CTX1-R6K-F" sequence="CGTTCCACTGAGCGTCAGACC"><BindingSite location="335-344" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="335-344" bases="GCGTCAGACC"/></BindingSite><BindingSite location="1646-1666" boundStrand="1" annealedBases="CGTTCCACTGAGCGTCAGACC" meltingTemperature="63"><Component hybridizedRange="1646-1666" bases="CGTTCCACTGAGCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="335-344" boundStrand="0" annealedBases="GCGTCAGACC" meltingTemperature="40"><Component bases="CGTTCCACTGA"/><Component hybridizedRange="335-344" bases="GCGTCAGACC"/></BindingSite><BindingSite simplified="1" location="1646-1666" boundStrand="1" annealedBases="CGTTCCACTGAGCGTCAGACC" meltingTemperature="63"><Component hybridizedRange="1646-1666" bases="CGTTCCACTGAGCGTCAGACC"/></BindingSite></Primer><Primer recentID="38" name="pMT494-F" sequence="TATCAGCTCACTCAAAGGCGgtggtataatatctttgttcattag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="0-44" boundStrand="0" annealedBases="TATCAGCTCACTCAAAGGCGgtggtataatatctttgttcattag" meltingTemperature="65"><Component hybridizedRange="0-44" bases="TATCAGCTCACTCAAAGGCGgtggtataatatctttgttcattag"/></BindingSite><BindingSite location="1836-1860" boundStrand="0" annealedBases="gtggtataatatctttgttcattag" meltingTemperature="51"><Component bases="TATCAGCTCACTCAAAGGCG"/><Component hybridizedRange="1836-1860" bases="gtggtataatatctttgttcattag"/></BindingSite><BindingSite simplified="1" location="0-44" boundStrand="0" annealedBases="TATCAGCTCACTCAAAGGCGgtggtataatatctttgttcattag" meltingTemperature="65"><Component hybridizedRange="0-44" bases="TATCAGCTCACTCAAAGGCGgtggtataatatctttgttcattag"/></BindingSite><BindingSite simplified="1" location="1836-1860" boundStrand="0" annealedBases="gtggtataatatctttgttcattag" meltingTemperature="51"><Component bases="TATCAGCTCACTCAAAGGCG"/><Component hybridizedRange="1836-1860" bases="gtggtataatatctttgttcattag"/></BindingSite></Primer><Primer recentID="39" name="pMT494-R" sequence="cttccagagtaaaaaccattataacctctccttaatttattgcatc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"><BindingSite location="273-318" boundStrand="1" annealedBases="cttccagagtaaaaaccattataacctctccttaatttattgcatc" meltingTemperature="63"><Component hybridizedRange="273-318" bases="cttccagagtaaaaaccattataacctctccttaatttattgcatc"/></BindingSite><BindingSite simplified="1" location="273-318" boundStrand="1" annealedBases="cttccagagtaaaaaccattataacctctccttaatttattgcatc" meltingTemperature="63"><Component hybridizedRange="273-318" bases="cttccagagtaaaaaccattataacctctccttaatttattgcatc"/></BindingSite></Primer></Primers>
  `<Notes>
<UUID>08da59e1-80b9-40e0-bb4c-0b5e0d40b1b8</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="11:5:38">2022.11.21</Created>
<LastModified UTC="3:35:8">2024.12.18</LastModified>
<CreatedBy>llllll</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y    K         
Unique Cutters                                                                                               d     CHO                E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
